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New Nano-materials As Applied to Sensing-interface Fabrication for Electrochemical Biosensors

Author: LiZheJian
Tutor: LiuZhiMin
School: Henan University of Technology
Course: Analytical Chemistry
Keywords: Electrochemical biosensor DNA Aptamer Nano- MnO2 Nano-ZnO E. coli Human immunodeficiency virus Adenosine
CLC: TP212.3
Type: Master's thesis
Year: 2010
Downloads: 49
Quote: 0
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Abstract


Electrochemical biosensor developed rapidly in recent years a new biosensor. Type of sensor has the advantages of high sensitivity, low cost, and good selectivity, easy miniaturization, have broad application prospects in the field of clinical examination, environmental monitoring and food engineering. Fixed to the base body above electrochemical biosensor constructed in a key technology is how the biological component is stable and efficient. The purpose of the research papers through the development of new biomaterials fixation methods, constructed a series of good performance of electrochemical DNA biosensors and aptamer sensors in order to achieve the improved the fixed biological component activity, and to extend the life of the sensor. This thesis research include: 1, room temperature solid legal system Preparation of nano-MnO of 2 , chitosan (CHIT) film-forming effect the nano of MnO 2 fixed in the surface of a glassy carbon electrode. DNA in MnO 2 / CHIT immobilization and hybridization of the membranes were characterized by cyclic voltammetry and electrochemical impedance. DNA probe with the target DNA is fixed to the surface of the electrode after hybridization of the electrode surface of the electron transfer resistance increases, as a detection signal may be a high sensitivity determination of the target DNA. Electrochemical impedance spectroscopy detection of E. coli gene fragment linear range of 2.0 × 10 -11 mol / L ~ 2.0 × 10 -6 mol / L, and the detection limit of 1.0 × 10 -12 mol / L. To room temperature solid-phase method to prepare nano ZnO CHIT film-forming effect of nano ZnO fixed in the surface of the glassy carbon electrode prepared ZnO / CHIT / GCE electrode to become a good platform for DNA immobilization and hybridization. Label-free electrochemical impedance detected by the human immunodeficiency virus (HIV) gene fragment linear range of 2.0 × 10 -11 to 2.0 × 10 -6 mol / L, the detection limit of 2.0 × 10 -12 mol / L. 3, by the deposition effect of the CHIT adenosine aptamer fixed in MnO 2 / CHIT increased the electron transfer resistance of the electrode surface after the surface of the electrode, the adenosine aptamer with the target adenosine hybridization, change in impedance value of the before and after hybridization with the amount of adenosine in the sample is proportional to, and thus can be measured in the high sensitivity of the target adenosine. The electrochemical impedance spectroscopy detection target adenosine linear range of 1.0 × 10-8 ~ 5.0 × 10 -6 mol / L, the correlation coefficient r = 0.9990, and the detection limit of 1.0 × 10 -9 mol / L. 4 of MnO 2 for sensing substrate constructed based on target-induced aptamer replacement unlabeled electrochemical adenosine sensor. Redox probe [Fe (CN) 6 ] 3-/4- the monitoring sensor interface surface electron transfer resistance changes. Application of electrochemical aptamer sensor for label-free detection of adenosine, electrochemical impedance spectroscopy, its linear range of 1.0 × 10 -9 to 1.0 × 10 -6 mol / L, the detection limit of 8.0 × 10 -10 mol / L.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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