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High salt environment Qijiaojing Salt Lake halophytic bacteria for the study, to explore the diversity of addicted to salt (resistant) bacteria and enzyme production characteristics. Acquisition Qijiaojing Salt Lake 15 soil samples chosen isolation medium separation addicted to salt (resistant) bacterial strains, application of multiphase classification techniques to identify its taxonomic status, phylogenetic tree based on 16S rRNA gene sequence analysis seven horns and well salt lake salt endophytic bacteria species diversity, functional enzyme screening, evaluation of production amylase and protease activity of production potential, as a culture addicted to (resistance) the development and utilization of the resources of the salt bacteria provide a theoretical basis and experimental data support. Isolated from soil samples in bacterial strains of the 15 copies Qijiaojing Salt Lake purified shape to repeat a total of 86, based on 16S rRNA gene sequence analysis identified to the genus level level. The distribution of 86 strains obtained by the separation in a thin-walled Bacillus the genus (Gracilibacillus) salt Xanthomonas (Halomonas), genus Bacillus (Bacillus) Chelativorans, the color salt genus Bacillus (Chromohalobacter), Tenuibacillus Thalassobacillus Halophila Bacillus Bacillus genus (Halobacillus), the sea spp (Marinobacter), Staphylococcus (Staphylococcus), the Bacillus brevis to genus (Brevibacillus), Solibacillus, Catellibacterium 13 genera. Which, Halobacillus case 30 17, Halomonas genus, Bacillus genus 13, Gracilibacillus is 9, Thalassobacillus in the case of five, Tenuibacillus in the case of 4, Catellibacterium case 2, Chelativorans is a, Chromohalobacter case 1, Marinobacter genus strains, Staphylococcus genus a genus 1, Brevibacillus, Solibacillus is a. The results show that, Halobacillus is absolute advantage in the number. Preliminary analysis showed that 37 strains of the 16S rRNA gene sequence similarity less than 98.5%, which initially determined to carry out the polyphasic taxonomic research to determine the explicit system taxonomic status as a candidate for a new species, it is worth. Multiphase classification method, the morphological characteristics of the strain YIM 93003 and YIM 94343 cell chemical components, G Cmol% of the 16S rRNA gene sequence phylogenetic analysis of their genomic DNA-DNA homology determination The results show that the strain YIM 93003 and YIM 94343 represents a different species of the genus Halomonas and named Halomonas qijiaojingensis sp nov., the Halomonas flava sp. nov. Screening of bacterial strains capable of growth in 10% NaCl under conditions to do the production of functional enzyme strain activity screening. The results showed that 18 strains of amylase activity in 18 strains of protease activity, strains containing the activity of two enzymes, and another eight were negative bacteria for the activity of these two enzymes. For the production of protease and amylase strains, Halobacillus in the case showed great superiority, which provide a favorable scientific basis for salt environment for the high activity of protease and amylase strains. The results show that, Qijiaojing Salt Lake culturable the halophyte bacteria not only rich in species diversity, but also has great potential for producing protease and amylase halophytic bacteria the use of resources in the future to provide a favorable scientific basis.
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