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Plants living environment is constantly changing, in order to produce offspring growth and reproduction, for different environmental factors will be felt in different ways, identify, and make the appropriate physiological responses, and ultimately adapt to the environment. If climate change is too violent, beyond the normal growth and development of plants that can tolerate limit, it will produce stress on plants. Plant Stress is usually mainly refers to abiotic stresses, including heat, cold, oxidative stress, drought, high salt and low potassium stress. Soil salinization affecting crop growth and yield of the most important environmental factors, resulting in a large number of crop failures, so the mechanism of plant salt tolerant crop varieties and cultivation is very important. Because of the regulatory network of plant salt tolerance is not very clear to understand and therefore needs further looking plant genome a gene related to salt tolerance, and to study the function of these genes. In this study, three different T-DNA activation tagging mutant library was screened salt and salt-sensitive mutants in order to explain the mechanism of plant salt to provide a theoretical basis. Three different activation tagging mutant library were: common feature to get type activation tagging mutant library, activation tagging plasmid vector as pSKI015, select labeled herbicide resistance; β-estradiol-induced activation tagging mutant library , activation tagging is a plasmid vector pER16, on which there XVE expression system selected kanamycin resistance marker; functional mutant gain-active tag library, a plasmid vector for the activation tagging pSKI074, selection marker is kanamycin resistance . Common features for gain-activation tagging mutant library and β-estradiol-induced activation tagging mutant library of 5,000 strains were resistant to high salt mutants screened and salt concentration of 200 mmol / L NaCl, screening Stress indicators in this wild-type Arabidopsis seedlings root growth was inhibited, just do not bend, you can finally get three generations of genetic mutants to T2, but the phenotype can not continue to be passed on to the next generation. Get type of functional activation tagging mutant library of 1000 lines were carried out with high salt Potassium salt-sensitive potassium mutants, hypokalemia medium potassium ion content of 40μmol / L, respectively, salt screening to 150 mmol / L and 50 mmol / L NaCl, screening indicators: low potassium medium 150 mmol / L NaCl concentration of wild-type Arabidopsis seedlings root bending does not occur the initial concentration; hypokalemia medium 50 mmol / L NaCl concentration of wild-type Arabidopsis root bend maximum concentration level, can not obtain high and stable inheritance of the potassium salt and potassium salt sensitive mutants. Although the final did not get stable genetic mutants, but the work of this thesis was to further screening other mutant plants provides an important reference.
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