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Research of Cell Capture, Manipulation and Optical Detection Platform

Author: HuRuiJun
Tutor: ChenDiï¼›ChenXiang
School: Shanghai Jiaotong University
Course: Microelectronics and Solid State Electronics
Keywords: Microfluidic Magnetic cell capture Cell micro- manipulation Capacitive micromachined force sensor Photodetector
CLC: Q2-33
Type: Master's thesis
Year: 2011
Downloads: 125
Quote: 0
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Abstract


Cell research for cancer, AIDS and other major diseases, research is very important to provide a convenient, fast cell capture and detection methods will facilitate the development of cell biology . MEMS technology will be used for basic research and cell biology miniaturized biological instrumentation study is significant . This article MEMS technology making use of a technology based on micro- bead cell capture chip, presents a cantilever control , capacitive mechanical testing cell operating platform, and developed a method for the optical detection of cell growth environment detection system , the contents include: 1 . introduction bead capture theory, the MEMS chip structure model and using simulation software to simulate the performance of the chip ; using MEMS fabrication process cell capture chip, using colon cancer cells and CD4 cells on the chip function test and get colon cancer cells in 0.1 ml / h flow rate capture efficiency reached 92.3% . 2 Using MEMS fabrication process for cell micro- manipulation power MEMS capacitive detection , the use of simulation software to simulate its mechanical and electrical properties , and to build a micro-capacitance measurement circuit . 3 Use the microcontroller , Arduino development board , VB procedures and structures such as LED and PD shimmer detection for cell growth environment miniaturization transmitted light detector system ; environmental testing the system and Blu-ray signal test , carried out using the yellow dye concentration testing. In this paper, the chip has a trace of cell capture and ease of operation, etc. ; using MEMS capacitive force-sensitive micro- force sensor control will be used for cell micro- cantilever platform is easy to operate cell research ; while LED-based and PD study cellular environment detection platform to be able to promote cell testing equipment miniaturization . The research on the cell biology of basic research and the development of clinical testing has important significance.

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CLC: > Biological Sciences > Cell Biology > Cell Biology Research Methods > The cytology experimental and cytological studies
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