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The Microbial Assemblages and Identification of Novel Functional Genes in Petroleum Reservoirs

Author: GaoCaiXia
Tutor: MouBoZhong
School: East China University of Science and Technology
Course: Physical and chemical
Keywords: assA gene Archaea Bacterial Microbial diversity Reservoir
CLC: TE39
Type: Master's thesis
Year: 2011
Downloads: 86
Quote: 0
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Abstract


The reservoir is a special environment , which gave birth to study microorganisms and their function has important theoretical and practical values ??. Molecular methods can be used as an effective means of in-depth understanding of the reservoir microorganisms . In this paper, the 16S rRNA gene library analysis of microbial community composition of the six blocks of samples of the three oil fields in China's onshore . The results show that the environmental samples of reservoir there is a wealth of micro - organisms , including archaea Most of the methane-producing bacteria , a few archaea (Themococcus, Halogeometricum and Thermogymnomonas ) also detected a small number of new archaea Uncategorized . The Methanogens mainly including methyl nutritional type (Methanomethylovorans), acetic acid nutritional type (Methanosaeta) and hydrogen nutrition methane-producing bacteria ( Methanothermobacter Methanolinea , Methanoculleus , etc.) , in which hydrogen nutrition methanogenic bacteria abundance . The bacteria , Proteobacteria highest frequency of occurrence , abundance , Firmicutes, Bacteroidetes, Actinobacteria and Thermotogae also occupy a certain proportion . Comprehensive analysis of the microbial composition of the reservoir environment relations that the reservoir temperature affect the composition of the microbial significant : high temperature reservoir to a high temperature bacteria , in the low-temperature reservoir thermophilic bacteria and mesophilic bacteria exist ; salinity reservoirs bacteria the type distribution there are some temperature under similar conditions , the high salinity of the reservoir types of bacteria are generally less rich reservoir of low salinity , bacteria type . For four wells produced water samples, the use of the alkyl succinic acid synthase gene ASSA - subunit ( ASSA ) target fragments of 523 bp of the functional gene primers detected the diversity of the gene , the establishment of assA functional gene library , found the new assA gene and distant the branch class assA gene . These results indicate that the reservoir of petroleum hydrocarbons in the presence of fumaric acid and bioactivation anaerobic degradation bacteria . These bacteria not belonging to a known containing assA gene flora , and is currently unable to determine their classification . This study will help to enrich the understanding of the reservoir environment , the flora and reservoir anaerobic degradation process .

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