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Biodegradation of the Lignocellulose Derived Inhibitor Substrances in the Pretreatment Process and Its Photo-fermentation for Hydrogen Production

Author: ZhuZhiZuo
Tutor: BaoJie
School: East China University of Science and Technology
Course: Biochemical Engineering
Keywords: Lignocellulose Pretreatment Inhibitor Biological detoxification Biological hydrogen production
CLC: TQ116.2
Type: Master's thesis
Year: 2011
Downloads: 130
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Abstract


Lignocellulose in the pretreatment process , furfural , hydroxymethyl furfural , acetic acid , formic acid , levulinic acid and other degradation products for subsequent enzymatic hydrolysis and fermentation process to produce a strong inhibition . The removal of these inhibitors , i.e. a so-called \The biological detoxification by microbial degradation is one of the most effective method . Through the synthetic medium screening and pretreatment of straw rescreening sieve out the three which can degrade the inhibitor of fungal pretreatment of lignocellulosic the : Amorphotheca resinae ZN1 . , Penicillium polonicum ZN2 . , Penicillium turbatum ZN3 and with 16sDNA method of strain the attributes of a preliminary identification . Growth Characteristics of experimental results show that , under conditions of high inhibitor concentration , A. resinae ZN1 at pH 3-8 , temperature 20-32 ° C under normal growth , and the oxygen demand is not strict . The bacteria concentration on the maximum degradation of age inhibitor exists independently Titanium 8g / L , 13g / l formic acid , furfural 4g / l, hydroxymethyl furfural 5g / l . Processed under typical dilute acid pretreatment conditions corn stalks , in a solid content of 30% under the conditions of the main components of the hydrolyzate after enzymatic hydrolysis : glucose 65 g / L , xylose 34g / L acetic acid 9.0g / l , 2.7g of formic acid / l , furfural and hydroxymethylfurfural 0.8g / l. The Saccharomyces yeast Saccharomyces cerevisiae DQ1 not without any detoxification hydrolyzate at 37 ° C under normal growth , while the preconditioning the ZN1 detoxification 4 days after A. resinae and hydrolysis straw , S. cerevisiae DQ1 under the same conditions the normal simultaneous saccharification and fermentation , the final ethanol concentration of up to 43g / l . Another part of the contents of this thesis is the use of sources of lignocellulose fermentation inhibitor as well as organic acids produced by the fermentation process of photosynthesis fermentative hydrogen production . Selection of Rhodobacter sphaeroides ZX - 5 strain for the the hydrolyzate fermentation of lignocellulosic grease photosynthetic organic acid fermentation hydrogen production . Experimental results show that the light intensity of 30 ° C , 45001ux R. sphaeroides ZX - 5 can be complete degradation of waste organic acids and residual sugar fermentation , hydrogen yield a 8ml H2/ml effluent . Organic acids utilized photosynthetic fermentation hydrogen production can not only solve the biofuel industrial wastewater treatment , and can produce clean hydrogen energy .

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CLC: > Industrial Technology > Chemical Industry > Basic inorganic chemical industry > Industrial gases > Hydrogen
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