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Pear (Pyrus bretschneideri Rehd.cv.Yali) originating in Hebei, is the oldest high-quality high-yielding varieties. Pear and robust, but the weak capacity of anti-scab. Pear scab is the major disease of pear of pear planting areas, often causing serious losses to the pear production has become a major limiting factor affecting the production of pear. Therefore, new varieties of breeding resistance to pear scab pear quality variation solve pear scab in the most economical, most effective and safest method. Pear history of cultivation for millennia ago, in the long-term cultivation process will spontaneously generate new variation type. Pear Hc suspected the ordinary pear mutant of new varieties of pear Hc mother tree and its branches were grafted grafted tree, observed by the field for several years, showed a high resistance to scab. In this study, pear Hc material identified by the field of anti-scab, pear Hc anti-scab disease-resistant types, and then from leaf morphology, physiological and biochemical indicators and molecular biology its antiviral mechanism system. Meanwhile, preliminary exploration pear scab fungus tissue culture in vitro culture and pear Hc stem segments. The main findings are as follows: 1. Applications vitro culture of different hormones and vitamin pear scab. The results showed that the α-naphthaleneacetic acid ,2,4-D, kinetin, 6-BA, vitamin B1 and vitamin C on the of pear scab mycelial growth with the promotion of the role of which the α-naphthylacetate promote role most significant. α-naphthaleneacetic acid added concentration of 10 mg / L. 2. Pear Hc perennial tree the Dongjian dormant buds branches, indoor hydroponic germination pumping raw spears and then germination spears as explants for tissue culture, the results show that the acquisition of Spear in mid-May for a suitable explants. Buds start medium for 1/2MS 6-BA0.2mg / L IBA 0.3 mg / L,. 3 naturally through the field-induced method, scab-resistant identification analysis pear Hc, the results show that the pear Hc control (pear) scab resistance to the presence of significant differences. Pear Hc belonging to the high resistance to scab type, the ordinary pear is highly susceptible to scab type. Disease-resistant pear of Hc and control (common pear) the different maturity blades organizational structure, including the total thickness of the blade, the thickness of palisade tissue, spongy tissue thickness, the thickness of the upper and lower epidermis, tissue tightness and porosity, wax content, stomatal density, leaf proportion of a comparative study. : Disease resistance pear Hc leaves ordinary pear the epidermis high wax content, stomatal density is low, the palisade tissue closely arranged and thick, compact structure of the gate the sea than high and leaf cells. Blade structure characterized by resistance to scab and pear certain correlation exists no correlation; leaf weight and disease resistance. Comparative study found that pear leaves his childhood Ye Zhicheng cooked leaves of resistant pear of Hc and control (ordinary pear) of different maturity Health blades the biochemicals content, including chlorophyll, total soluble sugar, soluble protein, disease-resistant pear the Hc chlorophyll content, soluble protein content than ordinary pear, total soluble sugar content is lower than ordinary pear. Orthogonal design method on four factors affecting the pear Hc ISSR-PCR reaction (template DNA, primers, dNTPs, Taq DNA polymerase) 5 levels of optimized test data processing software PCR results with DPS 7.55 analyzed to determine the optimum reaction system suitable for the pear Hc ISSR-PCR as follows: 20 μl of the reaction system, 1 × Taq PCR buffer [1.5mmol L -1 Mg 2 |