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Palm sawfly bucket vegetable (Petasites tatewakianus Kitam.) Petasites genus Asteraceae. Genus Petasites global total of 18, of the distribution of species produced in the northeast, east and southwest, Petasites, the hair split Petasites and palm the sawfly bucket vegetables rich domestic resources. The palm the sawfly bucket dish domestic distribution Xiaoxing'anling Changbai Mountain region. Background: Petasites genus European bee bucket vegetable (Petasites hybridus) herbal medicine, has been in Europe for hundreds of years of application history. The roots of the carbon dioxide extract for the treatment of migraine headaches, urogenital tract spasm, asthma, allergic rhinitis. Sesquiterpene compound is considered to be its main active ingredient, modern pharmacological studies have shown that the Petasites the hormone (petasin) and the sulfur the Petasites prime (S-petasin) sesquiterpene compounds have anti-histamine, inhibiting white three the ene synthesis and reduce the role of intracellular calcium concentration, so as to achieve the anti-inflammatory, antispasmodic efficacy. Research literature and reported for palm sawfly bucket dish system at home and abroad less studied chemical constituents and pharmacological activities of palm the sawfly bucket dish, it is particularly meaningful for mining of Petasites genus resources. Research purposes: palm the sawfly bucket dish comprehensive system resources survey to study the chemical composition of the palm sawfly bucket dishes, combined with pharmacological experiments, clear of the active ingredient and effective parts; establish a rapid and accurate determination of active ingredients, lay the foundation for palm sawfly Doo Choi resources development and utilization, and cerebral ischemia therapy research and development of new drugs. Research content and methods: 1, palm sawfly bucket food resources survey. On our mountains Xiaoxing'anling, Changbai Mountains palm sawfly bucket dish resources comprehensive resources survey to obtain first-hand information and provide the material basis for the development and utilization of the the Choi resources for palm sawfly bucket. Palm sawfly bucket dish rhizomes chemical composition research: from its ethanol extract, solvent extraction, silica gel chromatography, recrystallization isolated and purified monomer compounds, the application of physical and chemical constants and spectroscopic methods (melting point determination , IR, 1H-NMR, 13C-NMR, HMBC, HMQC, COSY, NOSEY, EI-MS, ESI-MS) to identify its structure. The active ingredient in the study on the basis of the chemical composition of the compounds to the treatment of cerebral ischemia activity. 3, HPLC method was used to establish the method for the determination of the active ingredients in the palm sawfly bucket dish and its different origin, different parts (roots, petioles, leaves) to determine the content and provide the basis for the use of resources. Results and Discussion: 1, isolated from palm sawfly bucket dish roots for 14 compounds were identified of which 10, respectively, for the same Petasites lactone, the Petasites lactone - III-a, IVa, VIa Ⅰ a, B , D, L, β-sitosterol, stigmastanol alcohol. Which resolve the Petasites lactone - Ⅵ a via structure is a new compound, the rest in addition to Petasites lactone B, all for the first time from this plant were isolated. Petasites based on the lactone - the VIa brain ischemia protection activity conducted a preliminary study, the results show that it has a good brain ischemia protected activity. 2, the domestic palm the sawfly bucket vegetables resources comprehensive resources survey in the northeast region of the distribution of resources collected six samples, respectively the Heilongjiang Province Raohe Rao Reclamation, of Shuangyashan small the best River, Yichun double Lalin Fung field, the Luobei the estuaries forest, Harbin Founder County, Jilin Baishan large mirror Gou, explore palm sawfly bucket dish intraspecific variation herbs genuineness and quality evaluation method to establish research provides a basis. 3, establish a HPLC method, determination of the different origin of the palm sawfly the bucket vegetables herbs Petasites lactone, the Petasites lactone - III a, Ⅳ and Ⅵ a, I a, B, D, L eight lactones The content of the component. The measurement results show sesquiterpene lactone content between origin have significant differences, and the origin of the river to the Shuangyashan small good content is the highest. This method provides the basis for the quality standards of the active ingredients of palm the sawfly bucket dish.
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