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Analysis on Nutritional Composition of Salicornia and Studies on the the Physiological Functions of Salicornia

Author: SongCaiXia
Tutor: ChenMeiZhen
School: Shantou University
Course: Biochemistry and Molecular Biology
Keywords: Salicornia Nutrients Polysaccharide In vitro antioxidant Lose weight Hypolipidemic
CLC: S649
Type: Master's thesis
Year: 2010
Downloads: 88
Quote: 0
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Abstract


This article was first planted in Nanao Island, Shantou, Guangdong Province, sea vegetables samphire, Salicornia young stems and seeds nutrients optimized spears extraction process of polysaccharides and polysaccharide extracted separation Purification and infrared spectroscopy, spears vitro antioxidant effects, weight loss hypolipidemic effect physiological activity and food security. Nutrition Analysis: The results showed that the of the fresh samphire the spears crude protein, crude fat, crude fiber, total sugars, polysaccharides, ash content (%): 1.34,0.28,0.76,1.28,0.39,3.42, vitamin C, β - carotene and the mineral elements content higher than ordinary vegetables, and rich body eight essential amino acids and umami amino acids, chlorophyll content up to 44.29mg/100g its high nutritional value and delicious; the samphire seed protein (34.01% ) and grease (23.79%) have a high content; range of seed protein amino acids, and contains eight essential amino acids, the seed oil is rich in unsaturated fatty acids, especially linoleic acid content of up to 70.62%, and is rich in vitamin E, its very high nutritional value. The samphire Spear heavy metals cadmium, mercury, lead and arsenic content were: 0.072, lt; 0.01,0.0279,0.0099 (mg / kg), below the national standard, with food hygiene requirements. The acute toxicity test results show that: Salicornia spears LD50 gt; 464g/kg, the effective non-toxic class (LD50 gt; 10g/kg) vegetables, the food safety. The Salicornia spears polysaccharide extraction conditions were optimized results for: water feed ratio of 48:1, extraction time 4.0h, extraction temperature 90 ° C, the maximum extraction rate under the condition predictive value of 2.01%. Two single polysaccharide component apex-Ⅰ apex-Ⅱ, apex-Ⅰ double distilled water elution fractions of polysaccharides were isolated and purified by DEAE-52 anion-exchange chromatography and Sephadex G-25 column chromatography, apex - Ⅱ 1mol / L NaCl solution gradient elution fractions. Analysis of infrared spectra, the initial guess the apex-I is a neutral polysaccharide having a β-D-pyran-glucose with an α-D-pyran-xylose structure, apex-Ⅱ containing a sulfuric acid group and the α-D-galactopyranoside lactose acidic polysaccharide structure. Salicornia spears extracts from antioxidant experimental results show: the samphire water extract, ethanol extract and water extract polysaccharides have some reducing power and antioxidant effects, effects were lower than the control, vitamin C, which The aqueous extract of the reduction of capacity and the ability to clear the · OH than the alcohol extract, but not display the clear O 2 - · of activity; the samphire crude polysaccharide and fine polysaccharides are clear · OH, a certain concentration range, the crude polysaccharide stronger activity than refined polysaccharide O 2 - · activity, but there was no clear. Weight loss nutrition obese mouse model experiments, the results show: the Salicornia spears significant inhibition of the increase in the weight of obese mice, reduce body fat mass, reducing the size of fat cells, and has a good weight loss. Salicornia stem juice, stems slag and stem hyperlipidemia obese rat model of lipid-lowering diet experiment results show that: Compared with model group, the weight loss of the whole stem strongest, could significantly inhibit weight gain in rats (p lt; 0.01), reduce obesity index (p lt; 0.01), lower fat index (p lt; 0.01) and decrease the volume of fat cells; addition, the lipid-lowering effect of the whole stem is the best, can be significant reduction in TG (p lt; 0.05), TC (p lt; 0.01) content and increased HDL-C level (p lt; 0.01). Visible, Salicornia Spear is better to lose weight and lipid-lowering effect, the effect of the whole stem.

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