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The Safety Evaluation of the Mitigate Wrinkle Prescription and the Whitening Prescription Whitening of the Moisturizer Emulsion

Author: ZhangShuai
Tutor: YangZhiBo
School: Hunan University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Whitening moisturizing lotion Safety Evaluation Skin allergy test Proliferation of fine fat Skin irritation test Keratinocytes
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 249
Quote: 0
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Abstract


Objective: To observe the whitening moisturizing lotion wrinkle removal formula and whitening formula of human keratinocyte proliferation activity; stimulation of guinea pig skin and white rabbits, immune response and pathological changes of the white rabbit skin. Method: 1, skin allergy test: guinea pig back hair removal after 2d, 7d and 14d, whitening moisturizer wrinkle removal formula and whitening formula coated on the back skin of guinea pigs maintain 6h, induced contact last induction 14d coated wrinkle removal whitening moisturizing lotion recipe and whitening formula in the dorsal skin of guinea pigs maintain 6h, excited with the contact. 24h and 48h excited after contact guinea pig body weight changes observed in guinea pig skin reaction and to compare the before and after testing. 2, skin irritation test: whitening moisturizer wrinkle removal formula and whitening formula is painted in white rabbit back skin after hair removal, maintaining 4h, 1,24,48 and 72h observation medication District skin reaction after drug is cleared. After consecutive 14d smear once a day, before each painting to remove residual drugs and observed at 1h after skin reactions take medication after the end of the experiment, the area of ??skin to do a biopsy to detect changes in the dermis and epidermis. 3, the cell proliferation assay: in vitro cultured human skin epidermal cells (HaCat) object wrinkle removal formula and whitening formula of different concentration of whitening moisturizing lotion after the cells were treated with the MTT assay at 492nm wavelength absorbance (A value) analysis whitening moisturizing lotion wrinkle removal formula and whitening formula HaCat cell proliferation activity. Results: 1, skin allergy test: Induction of the positive control group medication District of skin erythema, edema, negative control group and wrinkle removal formula group and whitening formula group skin did not change after exposure. Excited after contacting the skin of the positive control group medication District obvious erythema, eschar, edema, sensitization rate was 100%, no change in the negative control group and wrinkle removal formula group and the group of skin whitening formula, sensitization was 0.2, acute 1,24,48 and 72h skin skin irritation test: clear the test substance no obvious erythema, edema and reaction; several skin irritation test: consecutive 14d per day in clear residual test substance 1h observed skin whitening formula only group There are a erythema, no edema reaction an average score of 0.143. 3, histopathological examination: the whitening formula group and wrinkle removal formula group pathological Points are less than 4, no significant stimulation of the skin. Different concentration of whitening moisturizing wrinkle removal formula lotion and whitening treatment cells 12,24,48 h, the number of positive control cells significantly reduced, a large number of cell necrosis, disintegration; negative control group of cells with normal morphology increased significantly good growth; wrinkle removal formula group and whitening formula group normal most of the cell morphology, growth, individual cell necrosis, disintegration. Conclusion: 1, not only from the appearance, but also from the weight shown: whitening moisturizing lotion wrinkle removal formula whitening formula guinea pigs do not produce allergic skin sensitization. 2, not only from the appearance, but also from histopathological shows: the whitening moisturizer wrinkle removal formula and whitening formula no significant stimulation of the white rabbit skin. 3, by detecting holes in the 492nm wavelength absorbance (A value), wrinkle removal formula group and the group of IC50 whitening formula is obtained respectively 2.736mg/ml, 2.575 mg / ml. Cosmetics toxicity criteria under the EU National Laboratory, the whitening moisturizer wrinkle removal formula and whitening formula cytotoxic little or no cytotoxicity.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
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