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Diversity of Antagonistic Bacteria Isolated from Several Cash Crop Rhizosphere

Author: XuYingYing
Tutor: DuBingHai
School: Shandong Agricultural University
Course: Microbiology
Keywords: Rhizosphere Antagonistic BOXAIR-PCR 16S rDNA Diversity
CLC: S182
Type: Master's thesis
Year: 2011
Downloads: 42
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Abstract


This article analysis, in order to better understand the diversity of the crop rhizosphere antagonistic bacteria isolated from the antibacterial spectrum of antagonistic bacteria in 55 of the 10 important economic crops such as cotton, tobacco, tomatoes, garlic, ginger, grapefruit crop soil biological control-borne diseases provide a theoretical basis, using a combination of physiological and biochemical characteristics BOXAIR-PCR of 16S rDNA PCR-RFLP and 16S rDNA sequence analysis methods such as analysis of their genetic diversity. The measurement results show antibacterial spectrum antibacterial spectrum of antagonistic bacteria isolated from different crop rhizosphere are quite different; the from garlic rhizosphere DYJL5 and the DYJL10, from sunflower rhizosphere DYJL9 and the DYJL-G, from chilli rhizosphere DYJL13 and DYJL14 of broader antibacterial spectrum, more than 10 kinds of pathogens antagonism antagonistic ability; while larger the antagonistic differences between the antagonistic bacteria from chilli rhizosphere of: which DYJL13 and DYJL14 broader antibacterial spectrum and DYJL12 only celery gray mold pathogen (Botrytis cinerea Pers) and pepper root rot fungus (Fusarium solani) weak antagonism. BOXAIR-PCR with a variety of genetic map types, rhizosphere tested the antagonistic bacterium similarity level of about 72% can be clustered into seven groups, 85% poly 25 groups; 72% level, from the cotton root interpersonal DYJL4, DYJL6, DYJL25, DYJL26, DYJL29, DYJL30 ~ DYJL35 addition to DYJL25 poly in group 1 outside all the rest of polyethylene in group 2; from tomato, sunflower, garlic and chili the crop rhizosphere DYJL-A the DYJL-B DYJL All-C, DYJL9, DYJL-G, DYJL5, DYJL10, DYJL12, DYJL13 and DYJL14 gathered in group 2 the; from tobacco rhizosphere antagonistic bacteria DYJL38 ~ DYJL56 in together in group 2, group 4, group 6 and group 7 . 16S rDNA PCR-RFLP analysis, all the strains tested in the 73% similarity level together copolymer in the level of 83% to 13 genetic groups, pepper rhizosphere antagonistic bacteria DYJL12, separate clustered into groups 10; groups 5, group 6, group 11, group 12 and group all from tobacco rhizosphere antagonistic bacteria DYJL38 ~ DYJL56, 13; Group 3 from cotton rhizosphere antagonistic bacteria DYJL26, DYJL28 DYJL30 ~ DYJL36 grapefruit rhizosphere antagonistic the bacteria DYJL21, DYJL23, DYJL24, ginger root occasion of antagonistic bacteria DYJL19 tomato roots occasion of antagonistic bacteria DYJL-A DYJL-B composition; group 2 by from eight kinds of economic crops (jujube, garlic, cotton, ginger, sunflower, tomato, tobacco and melon) rhizosphere antagonistic bacteria (DYJL2, DYJL5, DYJL6, DYJL16, DYJL9, DYJL-C, DYJL55, DYJL-G, DYJL-H and DYJL-I) 10. The results show that the antagonistic bacteria in the rhizosphere of genetic diversity, the Flora of the genus Bacillus (of Bacillus spp.), The Paenibacillus genus (Paenibacillus spp.), Bacillus brevis case (Brevibacillus spp.), Pseudomonas Cellular genus (Pseudomonas spp.) and Alcaligenes (Alcaligenes spp.). This article was first pointed out the Heights Bacillus (Bacillus altitudinis) the tobacco black shank (Phytophthora nicotianae) original tomato gray mold fungus (Botrytis cinerea) and watermelon Fusarium wilt (Fusarium oxysporum f sp niveum) pathogen antagonism.

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