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Optimazation of the Method to Determinate the Content of Mannitol and the Fermented Condition of Using Amillariella Mellea to Produce Mannitol

Author: FanShuJun
Tutor: ZhuXianFeng
School: Henan University
Course: Microorganism
Keywords: A. mellea Mannitol Spectrophotometry Fermentation conditions
CLC: TQ923
Type: Master's thesis
Year: 2011
Downloads: 69
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Abstract


A. mellea has important medicinal value, and mannitol content is a of A. mellea series products important quality indicators. Therefore, effective accurate measurement of the content of A. mellea fungus powder mannitol, and A. mellea produced mannitol fermentation conditions crucial significance. In this study, conducted in two parts. The first part to establish the method for determination of Armillaria mellea fungus powder of mannitol target by alcohol extraction, water extraction, comparison of ultrasonic disruption method, filtering out the optimal Armillaria mellea sinensis pretreatment method; through sample solution with concentrated hydrochloric acid were hot boiling water bath method remove interference of fructose to mannitol Determination; by spectrophotometry in-depth comparison of iodometric method, select the mannitol content determination method. The end result: water as solvent ultrasonic crushing A. mellea sinensis 80 ° C operating temperature, the relative alcohol extraction method and water extraction method can effectively improve mannitol measured values; concentrated hydrochloric acid and the sample liquid boiling water thermal effective to remove fructose to mannitol Determination interference; spectrophotometric determination of value relative iodometric method is more accurate in the case of the presence of glucose and other monosaccharides mannitol interference. Precision test, the coloring stability test, the test sample solution stability test, repeatability test verification and recovery test, the final establishment of Armillaria mellea sinensis pretreatment method for ultrasonic crushing, removal of fructose on nectar interference method the alcohol content measured for a sample solution with concentrated hydrochloric acid of the thermal method, the mannitol content of the preferred measuring method spectrophotometry. The second part of the goal to improve the content of Armillaria bacteria mannitol mellea fermentation medium and fermentation conditions were optimized. Carbon source, nitrogen source single factor test, the carbon and nitrogen ratio of screening tests, inorganic salts and VB1 and trace elements mixture orthogonal test, and eventually established the optimal fermentation medium, its ratio of: glucose: 2% , sweet potato powder: 2%, corn flour: 30 g, wheat bran: 20 g, KH2PO4: 0.3%, MgSO4? 7H2O: 0.2%, VB1: 5 mg, trace element mixture: 6 mL of water: 1000 mL; be screened on the initial pH of the culture medium, inoculum size, medium volume, temperature, speed, and eventually establish the optimum fermentation conditions: medium initial pH: 5, inoculum size: 6%, liquid volume: 60 mL, temperature: 25 ° C, speed: 160 r / min.

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CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Fermentation industry > Fermentation legal system of higher alcohols and polyols
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