Dissertation > Excellent graduate degree dissertation topics show

Effects of Exogenous Polyamines on Growth and Physiological and Biochemical Characteristics of Cucumber Seedlings under NO3- Stress

Author: WangXiuHong
Tutor: WangXiuFeng；YangFengJuan
School: Shandong Agricultural University
Course: Olericulture
Keywords: Polyamine Antioxidant enzymes Photosynthetic characteristics Nitrogen metabolism-related enzyme activity Polyamine metabolism enzyme
CLC: S642.2
Type: Master's thesis
Year: 2011
Downloads: 44
Quote: 0
Read: Download Dissertation

Abstract


Cucumber (Cucumis sativus L.) is the biggest one of the vegetable crops cultivation area in China, because of its root system is shallow, with a the hi fat without Naifei characteristics, prone to salt damage. Soil salinization cucumber production has become a major obstacle, one of the main stress anion NO 3 - . The study showed that, the exogenous polyamine (polyamines, PAs) can improve the resilience of the plant, to some extent alleviate abiotic stresses. Therefore exogenous PAs NO 3 - stress on cucumber seedlings growth and physiological and biochemical characteristics, has important theoretical and practical significance for guiding cucumber sustainable production. The subject on the basis of existing research, using the hydroponic method to explore 140 mmol · L -1 NO 3 - Stress exogenous PAs on cucumber seedlings growth and nitrogen metabolism enzyme activity, photosynthetic characteristics, antioxidant enzyme systems and polyamine metabolism such as impact, in order to lay the theoretical foundation and open up new ways to mitigate the facility soil salinization hazards. The main results are as follows: 1,140 mmol · L -1 the NO 3 - stress, exogenous adding different concentrations of Spd, Spm and Put can promote seedling growth, with increasing concentration of Spd, Spm and Put treatment, the cucumber seedling height, leaf area increase in value and the net photosynthetic rate was increased at first and then decreased. 140 mmol · L -1 NO 3 - stress compared to plus 1 mmol · L -1 Spd, Spm and Put 7 d after treatment, the cucumber seedling height, leaf area, the added value and net photosynthetic rate differences most significantly, this concentration of polyamines processing can significantly improve the cucumber seedlings to high concentrations of NO 3 - stress resistance; Spd, Spm, and Put concentrations up to 2 mmol · L -1 , the added value of cucumber seedling height, leaf area and net photosynthetic rate to decrease trend. In summary the results show that 140 mmol · L -1 NO 3 - Stress, plus a certain concentration of Spd, Spm and Put promote cucumber seedling growth, high concentration is not conducive to the growth of cucumber seedlings. 2, compared with 140 mmol · L -1 NO 3 - stress treatment, plus 1 mmol · L -1 Spd, Spm and Put 7 d after treatment, the leaves of cucumber seedlings NR of GOGAT and GDH activity was significantly increased; NO 3 - -N and NH4-N content significantly reduce the increase of soluble protein and proline content. It showed that exogenous Spd, Spm and Put enhance high concentrations of NO 3 - Cucumber Seedlings under nitrogen metabolism level, reduce its NO 3 - -N and NH4-N accumulation of salt ions through the accumulation of soluble protein and proline osmotic adjustment substance of cucumber seedlings to enhance osmotic adjustment ability, thus contributing to the growth of cucumber seedlings. 3,140 mmol · L -1 NO 3 - Stress, salt-tolerant varieties' Xintai Mici 'and salt-sensitive varieties' Shennong The five spring 'cucumber seedling growth was inhibited, plant height, leaf area decreases, shoot and root dry weight reducing. Seedling leaves Pn, Ci, Gs and Tr significantly decreased NO 3 - stress of two cucumber varieties photosynthesis inhibition are stomatal limitation, 'Shen Nong spring five' the degree of inhibition of photosynthesis to higher than 'Xintaimici'. NO 3 - Stress 'the Shennong spring and five' plant height increased value and the value of the degree of inhibition of the increase in leaf area greater than 'Xintaimici' plus 1 mmol · L -1 Spd 'Shennong spring five' role in promoting the growth of greater than 'Xintaimici'. 4,140 mmol · L -1 NO 3 - stress (T2) treatment 1 d after seedling leaves of two cucumber varieties CAT activity as well as MDA content significantly increased APX activity was significantly decreased; the salt varieties 'Xintaimici' cucumber seedlings of SOD and POD activity and CK difference was not significant. 9 d after treatment, in addition to the two cucumber varieties MDA content and 'Xintaimici' cucumber seedling leaf SOD activity increased significantly, two cucumber varieties CAT, POD and APX significantly reduced. Plus 1 mmol · L -1 DCHA (T3) can be further exacerbated NO 3 - stress hazards. T2 and T3 treatment conditions, exogenous Add 1 mmol · L -1 Spd (T4 and T5) can raise the varying degrees of two cucumber varieties blades SOD, CAT, POD, and APX activity and MDA and reduce membrane lipid peroxidation. 5,140 mmol · L -1 NO 3 - (T2) two cucumber varieties of Spm in leaves increased stress, Spd and Put decreased. NO 3 - Stress, adding 1 mmol · L -1 DCHA (T3) significantly reduce the two cucumber varieties leaves Spd and Spm contents ,; 'Xintaimici' leaves the Put content with extended processing time showed an increasing trend, and the trend of 'Shen Nong spring' leaves Put content. T2 and T3 treatment conditions, add 1 mmol · L -1 Spd (T4 and T5) can significantly improve the two leaves of cucumber varieties of Spd, Put and Spm contents the reduced DAO and PAO activity, exogenous promote the cucumber seedlings endogenous polyamine synthesis, to maintain polyamine metabolism balance.

Related Dissertations

  1. Drought Stress on the Osmanthus Growth and Physiological Ecology Effect,S685.13
  2. The Effect of Extreme Temperature on Photosynthetic Physiology, Antioxidant Enzyme Systems and ROS of Fingered Citron,Q945.78
  3. Quantifying the Responses of Weak Gluten Wheat Leaf Photosynthesis and Chlorophyll Fluorescence Parameters to Weak Light at Filling Stage,S512.1
  4. Studies on the Effects of Exogenous Nitric Oxide on Rice under Cadmium Stress,S511
  5. Tolerance of Tomato on Rare Earth Elements and Its Remediation Potential on Polluted Environment,S641.2
  6. Study on Shade Resistance of Chrysanthemum and Its Related Taxa Seedlings,S682.11
  7. Effects of the Cyclic Starvation-Refeeding on Growth Performance, Body Composition, Digestive Enzymes and Antioxidant Enzymes of Megalobrama Amblycephala Yih and Cyprinus Carpio Var.Jian,S917.4
  8. Photosynthetic Characteristics of pepc Transgenic Rice under Salt Stress,S511
  9. Effects of Different Rootstock Grafting on Yield, Quality and Seedling Calcium Chloride Tolerance in Tomatoes,S641.2
  10. Effects of Heat Shock on Antioxidant Enzymes in the Fifth Instar Larvae of the Silkworm, Bombyx Mori,S881
  11. Influence of Baicalin on the Heart and Kidney Injury Induced by Iron Overload,R285
  12. Study on Photosynthetic Characteristics and Fruit Growth for Early Walnut Main Varieties in Xinjiang,S664.1
  13. The Preliminary Study of Biological Effect of Static Magnetic Field on Hydra Magnipapillata and Dugesia Japonica,X174
  14. Synthesis, Characterization and Properties of the N,N’-bis(3-aminopropyl)-2-hydroxypropylamine Complexes,O621.13
  15. Synthesis of N,N’-bis(3’-aminopropyl)-3-hydroxypropyl-amine and Research on the Polynitrogen Complexes,TQ226.31
  16. Studies on Seed Germination and Salt-Resistance Mechanism in Seedlings of N. Tangutorum,S567.239
  17. The Research on Physiological Characteristic of Seeds and Seedlings of Sloanea Hemsleyana Rehd.Et Wils,S792.99
  18. Trifoliate orange rootstock ' Shiranui ' and ' citrus ' zinc Stress and physiological mechanisms,S666
  19. The Research on Water Stress and Nutrient Stress of Seedlings of Zanthoxylum Ailanthoides Sieb. Et Zucc,S573.9
  20. Enhanced UV-B radiation effects on Tobacco Discussion chemical species,S572
  21. Mutagenic Effect of Satellite Carrying for Trifolium Repens,S541.2

CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Melons > Cucumber
© 2012 www.DissertationTopic.Net  Mobile