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The Observation of Radiation-Induced Pulmonary Injury in Rats by Different Dose Fractionation

Author: Yuan
Tutor: LiuWenQi
School: Guangxi Medical University
Course: Radiation Therapy Oncology
Keywords: Transforming growth factor- β1 (TGF-β1) Tumor necrosis factor - α (TNF - α ) Angiotensin-converting enzyme (ACE) Radiation-induced lung injury Serum Enzyme-linked immunosorbent assay (ELISA)
CLC: R818.02
Type: Master's thesis
Year: 2011
Downloads: 38
Quote: 0
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Abstract


The purpose of establishing different dose fractionation mode irradiation radiation-induced lung injury in animal models of rats were irradiated right lung after different dose fractionation mode to detect the different periods of serum transforming growth factor β1 (TGF-β1), tumor necrosis factor-α (TNF-α), factor expression changes in the level of angiotensin-converting enzyme (ACE), the pathological changes of the dynamic observation of radiation-induced lung injury in rats, preliminary study of TGF-β1, TNF-α and ACE early acute radiation pneumonitis and late chronic pulmonary fibrosis different dose fractionation mode irradiation differences in radiation-induced lung injury. 90 Wistar rats were randomly divided into group A (small fractionated irradiation group), B group (Oita irradiated group), C (control group), using 60Co-γ ray irradiated rats right lung irradiation , Weekend rats the 1,3,5,10,24 after irradiation venous blood, serum TGF-β1, TNF-α, ACE content was measured by ELISA, and rat lung tissue were sacrificed at various time points, with HE staining pathological changes of lung tissue damage. Results. Oita irradiation serum TGF-β1 content starting from one week after radiotherapy, rapid linear growth, to 10 weeks, serum TGF-β1 content reached its peak, 5 weeks to 24 weeks maintained at a higher level; parabolic type changes of serum TNF-α levels after radiotherapy serum TNF-α levels increased rapidly from the lower level of the first week, peak to 10 weeks, after rapid decline; the serum ACE content was a V-type, that began one week after radiotherapy, serum ACE content decreased to 10 weeks to reach the bottom, and afterwards picked up rapidly; 2 small fractionated irradiation group different time periods serum TGF-β1, TNF-α, compared with the control group was higher, but is much smaller than elevated the Oita irradiation group; serum ACE in the first weeks after radiotherapy slight decrease to 10 weeks to appear quickly elevated higher than the normal group, and then back down to normal levels; 3. Oita irradiation serum ACE content below the small fractionated irradiation and control groups at each time point; small fractionated irradiation serum ACE only 10 week higher, remaining at each time point were lower than the control group; rat lung tissue after irradiation experienced a pathological injury from acute radiation pneumonitis to chronic pulmonary fibrosis, including acute inflammatory phase, the proliferative phase and Fibrosis period. Both acute radiation-induced inflammation or radiation fibrosis fractionated irradiation group larger the fractionated irradiation group was slightly; expression of TGF-β1, TNF-α levels in the serum with the degree of early acute radiation injury aggravated gradually increased, and in the most serious of the acute radiation pneumonitis, the highest expression levels. The late lung tissue gradually fibrosis process, TNF-α decreased rapidly, and the TGF-β1 remained at higher expression levels. Conclusion rat lung irradiation of TGF-β1 in serum TNF-α content changes with the degree of lung tissue damage is closely related to, and gradually increased with TGF-β1, TNF-α, the pathology of the lung tissue injury gradually worsened. TNF-α, TGF-β1 play an active role in the early radiation damage caused by acute inflammation, and the important role of TGF-β1 on late damage caused pulmonary fibrosis. In an interview with the same total dose irradiation of TGF-β1 in small fractionated irradiation serum TNF-α content increased rate was significantly lower than the the Oita irradiation group, and the degree of lung injury in rats significantly larger the fractionated irradiation group light, and thus the of small fractionated irradiation of the late response organizations have good protection. Irradiated rat lung tissue, serum ACE content early radiation-induced lung injury caused by acute inflammation were negatively associated with serum ACE content will also help in monitoring the late lung tissue in vascular endothelial cells in the repair of radiation injury situation. Every time the 2Gy small fractionated irradiation mode the irradiated rats unilateral right lung 30Gy safe.

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CLC: > Medicine, health > Of Medical > Radiation Medicine > Radiation sickness,radiation damage > Pathology
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