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Study on the Effects of Cognitive Function Caused by Hypoxic Injury in the Neonate Rats

Author: LuoBin
Tutor: ZhaoChunLing
School: Luzhou Medical College
Course: Physiology
Keywords: Chronic intermittent hypoxia OSAHS Brain damage EEG Morris water maze GAP-43 Neurogenesis Development
CLC: R766
Type: Master's thesis
Year: 2011
Downloads: 34
Quote: 0
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Abstract


Objective: To establish an early age and development of chronic intermittent hypoxia in rats brain injury model, using electrophysiological methods, methods of behavioral and histological methods, observe the pups in the early stages of growth and development, due to lack of oxygen lead to damage of the brain's neurons progressive change and cognitive function in chronic hypoxic brain injury due to resistance to change, and to explore the possible mechanism of its occurrence, cause cognitive dysfunction mechanism for further study of children with obstructive sleep apnea hypopnea syndrome (OSAHS) experimental basis. Method: 1, selected 96 male SD rats, 20 ± 2 days old, were randomly divided into six groups: normal 2 weeks, normally four weeks, six weeks of normal group, two weeks of intermittent hypoxia, lack of intermittent oxygen groups of four weeks, six weeks of intermittent hypoxia group, n = 16. Normal (UC) group of normal feeding and intermittent hypoxia (IH) group, to intermittent hypoxic injury model. 2, electrophysiological testing: modeling success, randomly selected from each group, 10 rats, frontal cortex EEG recording with a metal electrode. Behavior detection: after the success of the model, the Morris water maze determination of each remaining six rats learning and memory abilities. The morphological detection: cortical EEG detection after conventional rats were killed, HE staining observed changes in hippocampal CA1 region of conventional light microscopy. Results: 1, electrophysiological testing: two weeks of intermittent hypoxia rat frontal cortex δ wave compared to the normal group, the proportion of θ waves share increased proportion of α wave, β wave is reduced compared to the normal group, there were significant difference (P lt; 0.05); intermittent hypoxia 4 weeks and six weeks later, although the rat prefrontal cortex δ wave, compared to the normal group, the proportion of θ waves share has increased, α wave, β wave compared with the proportion The decrease in the normal group, but did not reach a significant difference (P gt; 0.05). Morris water maze academic performance (navigation): After five days to find a platform for training, each group continuously shortened escape latency of the overall trend. Repeated measures variance analysis results show: (1) between the different number of days to escape the incubation period significantly difference (P lt; 0.05); (2) between the two groups compare: IH2w group's escape incubation period is significant with longer than UC2w group (P lt; 0.05) IH4w group's escape incubation period is significantly longer than the UC4w group (P lt; 0.05) IH6w group's escape incubation period is significant with longer than UC6w group (P lt; 0.05); (3) group comparison: UC group with increasing age, evade incubation period gradually shorten each age group there is a statistically significant difference (P lt; 0.05); IH2w escape latency was significantly longer than IH4w group, and IH6w of group (P lt; 0.05), IH4w group escape latency longer than IH6w group, but the difference was not statistically significance (P gt; 0.05). Morris water maze memory scores (space search experimental results): (1) across the target quadrant time accounting for the entire swim time of Percentage: ① The group comparison: IH2w group compared with the UC2w group significantly significantly reduce (P lt; 0.05), IH4w group compared UC4w group a significant reduction (P lt; 0.05), IH6w groups representing UC6w group significantly reduced (P lt; 0.05). ② group compare: UC group leap the target quadrant Time total swimming time percentage gradually increased with increasing age, there is a significant difference among age groups (P lt; 0.05); IH2w group significantly the less than IH4w group, and IH6w of group (P lt; 0.05) IH4w group less than IH6w group, but the difference was not statistically significance (P gt; 0.05). (2) through the platform Views: ① The group comparison: IH2w group compared with the UC2w group significantly reduce (P lt; 0.05), IH4w group compared with the UC4w group significantly significantly reduce (P lt; 0.05) IH6w group compared with the UC6w group significantly reduce (P lt ; 0.05). ② group compare: UC group with increasing age, the gradual increase in the number of crossing the platform, there is a significant difference among age groups (P lt; 0.05); crossing the platform IH2w number of significant of less than IH4w group, and IH6w of group (P lt; 0.05), IH4w group escape latency less than IH6w group, but the difference was not statistically significance (P gt; 0.05). 3, HE staining light microscopy, visible group of normal rat brain hippocampal CA1 region structure hierarchy is clear, the nerve cells arranged in neat rows, normal morphology and nuclear membrane integrity, the nucleolus clear, no cell degeneration and necrosis. Hypoxic rats brain hippocampal CA1 region IH2w group the number of neurons decreased neuron cell body swelling, nuclear membrane is unclear, the nucleolus disappears visible degeneration and necrosis of the cells; IH4w group, and IH6w of cells swollen cell disorder, but milder than those IH2w group; IH4w group, and IH6w of groups compared in the light microscope, the difference is not obvious. Conclusion: Chronic intermittent hypoxia can cause abnormal changes in juvenile developing rat prefrontal cortical electrical activity. 2, chronic intermittent hypoxia can lead to a decline in childhood and development of cognitive function in rats. 3, chronic intermittent hypoxia causes the the juvenile developing rat hippocampal CA1 neurons morphological changes. 4, the juvenile developing rat brain damage caused by chronic intermittent hypoxia compensatory repair capacity.

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