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Objective: To investigate HPV infection and FHIT gene abnormalities and genital warts in the genital development of squamous cell carcinoma of the role and relationship. Methods: Polymerase chain reaction - single strand conformation polymorphism analysis (PCR-SSCP) detection vulvar squamous cell carcinoma, genital warts, vulvar tissue normal FHIT gene loci D3S1300 and D3S1481 loss of heterozygosity (LOH) and micro- instability (MSI); using polymerase chain reaction (PCR) detection of squamous cell carcinoma of the vulva, genital warts, vulvar normal tissues HPV6, 11,16,18,31,33 typing; against genital warts underwent partial electrocautery lesions treated with α-2b interferon gel, followed up for 3 months to observe the low-risk HPV infection group and the high-risk group of recurrence rate. Results: ① in the D3S1300 locus, LOH and MSI-positive rate in the normal vulva, VCA, VSCC 0%, respectively, 21.4% (9/42), 54.2% (13/24), there are differences among the three groups statistically significant (χ2 = 17.557, P = 0.000 lt; 0.05); VSCC and VCA, VSCC compared with the normal genital differences were statistically significant (χ2 = 7.366, P = 0.007 lt; 0.017; χ2 = 15.376, P = 0.000 lt; 0.017); VCA normal vulva, the difference was not statistically significant (Fhisher's = 0.047 gt; 0.017). ② In the D3S1481 locus, VSCC failure to provide information. LOH and MSI positive rate of VCA in the normal vulva and 0%, respectively, 19.0% (8/42), normal vulva and VCA difference was statistically significant (Fhisher's = 0.046 lt; 0.05). ③ In the D3S1300 locus, VSCC organizational HR-HPV infection and FHIT gene abnormalities related (r = 0.438, P lt; 0.05); VSCC organization LR-HPV infection and FHIT gene LOH / MSI no correlation (r = 0.158, P = 0.461 gt; 0.05). ④ In the D3S1300 locus, VCA organization LR-HPV and HR-HPV infection and FHIT gene LOH / MSI was no correlation (r = 0.169, P = 0.283 gt; 0.05; r = 0.027, P = 0.866 gt; 0.05). ⑤ In the D3S1481 locus, VCA organization LR-HPV and HR-HPV infection and FHIT gene LOH / MSI was no correlation (r = -0.049, P = 0.757 gt; 0.05; r = -0.194, P = 0.219 gt; 0.05 ). ⑥ genital warts in tissues, HPV-positive rate from high to low were HPV11 (90.5%, 38/42), HPV6 (88.1%, 37/42), HPV18 (23.8%, 10/42), HPV16 (7.1 %, 3/42), HPV33 (2.4%, 1/42), HPV31 (0%, 0/42). ⑦ in the vulvar tissue, HPV-positive rate from high to low were HPV11 (79.2%, 19/24), HPV18 (25.0%, 6/24), HPV33 (16.7%, 4/24), HPV16 and HPV31 ( were 12.5%, 3/24), HPV6 (8.3%, 2/24). ⑧ low-risk (LR-HPV, HPV6/11) positive rate in normal vulva, VCA, VSCC group was 10.0% (2/20), 90.5% (38/42), 87.5% (21/24) , three statistically significant difference between the groups (χ2 = 46.988, P lt; 0.001); normal vulva and VCA, normal vulva and VSCC, the difference was statistically significant (χ2 = 38.329, P lt; 0.001; χ2 = 26.263 , P lt; 0.001); VCA and VSCC, the difference was not statistically significant (Fhisher's = 0.699 gt; 0.017). ⑨ high-risk (HR-HPV, HPV16/18/31/33) positive rate in normal vulva, VCA, VSCC group were 0% (0/20), 30.9% (13/42), 41.7% (10 / 24), the difference between the three groups was statistically significant (χ2 = 10.409, P = 0.005 lt; 0.05); normal vulva and VCA, normal vulva and VSCC group, the difference was statistically significant (Fhisher's = 0.006 lt; 0.017 ; Fhisher's = 0.00 lt; 0.017); VCA and VSCC, the difference was not statistically significant (χ2 = 0.772, P = 0.380 gt; 0.017). ⑩ VCA in: the low risk group (ie, simple low-risk types HPV6 or 11 infection in 29 cases) and high-risk groups (ie, HPV16, 18,31 or 33 any type of infection 13 cases) recurrence rate was 27.6% (8/29) , 38.5% (5/13), low-risk and high-risk groups in the VCA group no significant difference in recurrence rate (Fhisher's = 0.495). High-risk group of patients were associated with CIN recurrence (3/3). Conclusion: ① FHIT gene LOH and / or MSI with the occurrence and development of VSCC. ② VSCC of HR-HPV infection and FHIT gene LOH and / or MSI-related. ③ VSCC, VCA are simultaneously present LR-HPV and HR-HPV infection complex. ④ no evidence that high-risk HPV types VCA low-risk HPV infection is easy to relapse, but high-risk group associated with a higher recurrence rate of CIN cases.
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