Dissertation > Excellent graduate degree dissertation topics show

1 colorectal serrated lesions of RUNX3 expression and gene polymorphism observational studies ; 2 serrated colorectal adenoma lint pathological and immunohistochemical characteristics

Author: WangLin
Tutor: WangLuPing
School: Shanxi Medical
Course: Pathology and Pathophysiology
Keywords: Serrated lesions Serrated adenomas Hyperplastic polyps Runx3 transcription factor Filiform serrated adenoma Non-Filiform serrated adenoma Hyperplasia
CLC: R735.3
Type: Master's thesis
Year: 2011
Downloads: 59
Quote: 0
Read: Download Dissertation

Abstract


[Background and Objective]Serrated lesions are a group of colorectal adenoma/polyps characterized morphologically by a serrated architecture of the crypt epithelium.Because of their malignant potential, serrated lesions become Research focus and get more attention by clinical Pathologist in recent years.The lesions include hyperplastic polyps(HPs),sessile serrated adenoma/ polyp (SSA/P) and traditional serrated adenoma(TSA) in WHO(2010).TSA and SSA have malignant potential,and different types of HPs also has similar genetic changes like TSA and SSA. Runt-related transcription factor 3 (Runx3) is considered to be a tumor suppressor gene, and have been found that there are RUNX3 gene abnormalities in variety human tumors in recent years. Studies have found that Runx3 is more closely related with human digestive system tumors, But the effection of RUNX3 gene in serrated lesions is not clear.RUNX3 is located on human chromosome 1p36.1,mainly express in gastrointestinal epithelium cells, blood cells, mesenchymal, nerve cells, etc. Research showed RUNX3 could inhibit growth of tumors by regulate cell cycle and induce apoptosis of tumor cell. In this study,the immunohistochemical expression and genetic polymorphism analysis of RUNX3 were observed in serrated lesions, the relationship between them were discussed, and the effection of RUNX3 in serrated pathway were explored.[Methods]A total of 5347 cases of colorectal polyps from five hospitals during a five-year period were retrospectively reviewed. The serrated lesions were classified based on WHO standards and literature. Amongst 5347 colorectal polyps studied,258 cases of serrated lesions were found. Amongst 258 cases of serrated lesions,32 cases of TSA, 8 cases of SSA/P and 25 cases of HP were selected. 25 cases of TA, 25 cases of normal colorectal tissues and 20cases of invasive adenocarcinoma were selected as the controls.All tissue sections were stainned using RUNX3 antibody by immunohistochemical methods.Besides, 50 cases of SA (including 40 cases of SA and 10 cases of SSA/P) and 20 cases of normal colorectal tissues were selected as the controls.Polymorphisms of RUNX3(rs2236851,C/T ) were genotyped by PCR-SSP. The relationship between immunohistochemical expression and genetic polymorphism were explored by IPP methods.[Results]The positive rate of RUNX3 in TSA,SSA and CRC were lower than in normal group.There were significant differences between the TSA,SSA,CRC and normal group.The significant differences also were observed between SSA,CRC and TA.The study of RUNX3 (rs2236851,C/T) polymorphism and the semi-quantitative analysis using IPP software was showed that the frequency of T allele was significantly higher in serrated adenoma(SA) than the normal control group. The RUNX3(rs2236851,C/T) was closely related with SA and the immunohistochemical expression of RUNX3 was lower in SA with TC genotype.[Conclusions]1. Immunohistochemistry expression results showed that the positive rate of RUNX3 expression is reduced in serrated lesions.It was a necessary events for SA changing into malignant lesions.2. The results of RUNX3 genetic polymorphism analysis suggest that RUNX3 (rs2236851, C / T) was closely related with the SA, the expression of RUNX3 in SA (TC genotype) was reduced. [Background and Objective]Serrated lesions are a heterogeneous group of lesions characterized morphologically by a serrated architecture of the epithelial compartment.The lesions include hyperplastic polyps(HPs),sessile serrated adenoma/ polyp (SSA/P) and traditional serrated adenoma(TSA) in WHO(2010). according to the type of mucus, HPs were divided into microvesicula HP (MVHP), goblet cellric HP (GCHP) and mucin poor HP ( MPHP). And according to cell atypia, the SSA/P were divided into SSA/P with dysplasia and SSA/P without dysplasia.Some studies reported an unusual type of TSA with long filiform projections lined by neoplastic epithelium with a serrated contour. These lesions were called Filiform Serrated Adenoma (FSA) in WHO( 2010). At present, domestic and international research with FSA is uncommon and the knowledge about these lesions was little understanding among clinical physicians and pathologists. Most of the pathological and clinical physicians to understand it even less.Therefore, this study discusses the clinical and pathological features of FSA and explore the immunohistochemical chang. Aim to help clinical pathologists enhanced understanding of FSA and to improve the management of patients.[Methods]A total of 5347 cases of colorectal polyps/adenomas from five regional hospitals in five years were retrospectively reviewed and 18 cases of FSA were selected. 20 cases of NFSA(including 15 cases TSA and 5 cases of SSA/P),20 cases of HP and 20 cases of VTA were randomly selected as control group. The clinical pathologic feature of FSA were observed.The expression of Ki-67, p53, CK7,CK20, CDX2, beta-catenin and RUNX3 in FSA,NFSA,HP and VTA were compared by immunohistochemical stain.[Results]Observation of clinical feature showed that FSA account for 0.34% of 5347 colorectal polyps/adenomas and account for 6.98% of 258 serrated lesions.FSA was tend to occur in older adults, and showed a strong predilection for developing in the left colon, especially the rectum. Observation of pathological features showed that FSA with long filiform projections lined by neoplastic epithelium with a serrated contour. The filiform projections of FSA were longer than the villi present in typical VTA .Marked stromal edema was present and the "bulb"-like change was showed in swelling of Severe cases. There was statistically significant difference between FSA and NFSA in the degree of dysphasia(p<0.05). The expression of Ki-67,p53,RUNX3 showed significant difference among FSA ,NFSA,HP and VTA (P<0.05); while the difference among expression ofCK7,CK20,CDX2 and beta-catenin in FSA ,NFSA,HP and VTA were not observed (P>0.05).[Conclusions]1. Observation showed that FSA is an uncommon type of serrated adenoma, shows a strong predilection located in the left colon,especially in rectum.2. Pathological observation results show that FSA has long filiform projections lined by neoplastic epithelium with a serrated contour unlike traditionalSA,.3. Immunohistochemical results suggest that FSA may has higher proliferation activity and higher malignant transformation potential.

Related Dissertations

  1. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  2. To Study Prognosis Molecular Marker of Prostate Cancer:P504S and B7-H3,R737.25
  3. RevoLix 2 micron laser treatment of benign prostatic hyperplasia clinical application,R697.3
  4. Inhibitory Effect of Ginsenoside Rb1 on Proliferation of Vascular Smooth Muscle Cells and Carotid Neointimal Hyperplasia Induced by Balloon Injury,R285.5
  5. Effect and Mechnisms of MIR-21 in Low Shear Stress Induced Carotid Remodeling in Mice,R363
  6. Expression of Tiam1 and KISS-1 in Esophageal Squamous Carcinoma and Its Significance,R735.1
  7. Effect of Rapamycin on Stromal Cell-derived Factor-1 Alpha Expression and Migration of Rat Vascular Smooth Muscle Cell,R96
  8. The Study of Adventitia Applied Slow-releasing Rapamycin Inhibiting Intima Hyperplasia of Vascular Grafts in Rabbits,R965
  9. Expression and the Elinieal Signifieanee of CD133、 CD44 and TROP-2 in the Prostate Cancer Tissues,R737.25
  10. Expression Level of NF-κB/p65, p16 and ki67 in Endometrial Hyperplasia with RU486 Treatment,R711.74
  11. A Comparative Study on the Results of Two Micron Laser Vaporesection of Prostate and Bipolar Transurethral Plasmakinetic Prostatectomy for Benign Prostatic Hyperplasia,R699
  12. A Comparative Study of 2μm Laser Vaporization and Resection of the Prostate, Greenlight Photoselective Vaporization of the Prostate and Transurethral Resection of the Prostate for BPH,R699
  13. The Inhibition Effects of Amlodipine Besylate on Neointimal Hyperplasia Induced by Balloon Injury in Rats,R96
  14. Study of the Association between Diabetes and Benign Prostatic Hyperplasia,R697.3
  15. The Study of Expression of S100B in Patient’s Bladder Detrusor with Benigh Prostatic Hyperplasia Combined Type 2 Diabetes Mellitus,R587.1
  16. The Value of DWI and 3D ~1H-MRS for the Differential Diagnosis of Beign and Malignant Disease of the Prostate,R697
  17. 2μm Laser Versus Transurethral Vaporization-Resection of the Prostate: Comparative Changes in Histopathological Features of the Resected Specimens,R699
  18. Construction and Identification of pExpress-1 Expression Vector for rHSG,R346
  19. Dung beetle effective substance against prostate hyperplasia basic research,R285
  20. Expression of bFGF and TGF-β1 in Benign Prostatic Hyperplasia Combined with Prostatitis and Their Significances,R697.32
  21. Experimental Study of Anti-inflammatory and Anti-intimal Hyperplasia Effects of p38MAPK Inhibitor CBS3830 in Arterialized Autogenous Vein Grafts in Rats,R654.2

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms
© 2012 www.DissertationTopic.Net  Mobile