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Objective To investigate the acute intestinal obstruction and bacterial translocation mechanism . Methods Polymerase chain reaction (PCR) qualitative detection of E. coli bacteria from the 16SrRNA and specificity galactosidase gene BG; mesenteric lymph nodes bacterial culture ; immunohistochemical detection of intestinal lamina propria T cell subsets , plasma cell count ; enzyme-linked immunosorbent assay (ELISA) quantitative detection of serum TGF-β1 concentration ; enzyme-linked immunosorbent assay (ELISA) detection of acute intestinal obstruction group before surgery, and the control group, serum interleukin -10 (IL-10 ) concentration . Results bowel resection anastomosis group 16SrRNA, BG positive rates were 86.4 %, 72.7% , lymph node culture positive rate of 77.3% ; Not bowel resection group 16SrRNA, BG positive rates were 64.3% , 42.8% , lymph node culture positive rate of 57.1% ; conservative treatment group 16SrRNA, BG positive rates were 57.1% , 28.5% , normal bowel serum control group 16SrRNA, BG was not detected ; obstruction intestinal resection and anastomosis group intestinal lamina propria T cell subsets , plasma cell numbers were low in the normal control group ; bowel resection anastomosis surgery group , not bowel resection group preoperative serum TGF-β1 levels were higher than the conservative treatment group and the control group , after 7 days of serum TGF-β1 concentration was no difference compared with the control group ; obstruction groups preoperative serum IL-10 concentration was higher (P lt; 0.01); each group preoperative serum concentrations of IL-10 have more differences (P lt; 0.05); obstruction preoperative and each group after there are differences (P lt; 0.01). Conclusion Acute intestinal obstruction in children with intestinal lamina propria T cell subsets , plasma cells were reduced, causing mucosal immune barrier damage, while TGF-β1 and IL-10 levels, participate in immune suppression, immune dysfunction , increased mucosal immune barrier damage, increase the body's susceptibility to promote bacterial translocation .
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