Th17 and CD4 IL-10 T cell function. Method: choose healthy controls HC 10, single house dust mite allergy " />
Dissertation > Excellent graduate degree dissertation topics show

Function Research of Th1/2、Th17 Cells and CD4~+ IL-10~+ Treg Cells of Peripheral Blood in AR and DE

Author: ZhangXuJing
Tutor: KongWeiJia
School: Huazhong University of Science and Technology
Course: Otorhinolaryngology
Keywords: Allergic Rhinitis Desensitization efficacy The dust mites stimulus allergen PMA Th17 Treg IL-10 Th1 Th2 Central memory T cells Effector memory T cells
CLC: R765.21
Type: Master's thesis
Year: 2011
Downloads: 21
Quote: 0
Read: Download Dissertation

Abstract


Objective: To investigate the peripheral blood of patients with AR and DE Th17' dissertation">Th17 and CD4 IL-10 T cell function. Method: choose healthy controls HC 10, single house dust mite allergy in patients with perennial allergic rhinitis AR 10, desensitization efficacy of good patient to DE 8 people. Extracted from peripheral blood mononuclear cells the layer (PBMCs) were divided into a control group, to dust mites group and the PMA group, blank group joined the ionomycin 1ug/ml and monensin 2ug/ml; dust mite group joined ionomycin 1ug / ml and monensin 2ug/ml, dust mites stimulus becomes allergen of nDerp1 1 ug / ml; the PMA group joined the ionomycin 1ug/ml monensin 2ug/ml, PMA 20ng/ml after 37 ° C and cultured in a 5% CO2 incubator 6h. Membrane and intracellular staining using flow cytometry detector test after PBMCs Th17 in the percentage of CD4 T cells, Th17 cell function analysis in peripheral blood of patients with AR and DE; detection of IL-10 in CD4 T cells Percentage of Results: HC blank group, dust mites group, PMA group the percentage of Th17 cells are lower than the corresponding AR group (P lt; 0.05), DE blank group, the group of dust mites are lower than the percentage of Th17 cells corresponding to the AR group (P lt; 0.05); stimulated by dust mites, AR compared with blank group decreased (P lt; 0.05); HC, AR and DE Th17 cell percentages were higher than the blank group (P lt; 0.05) after PMA stimulation. The blank group HC DE Treg cells secrete IL-10 were lower than the AR (P lt; 0.05); stimulated by dust mites, AR blank group to lower Treg cells secrete IL-10 (P lt; 0.05). Conclusion: Th17 cells may be involved in immune mechanisms of allergic rhinitis. PMA stimulation of Th cell percentage of IL-17 can be increased. Treg cells prompt the secretion of IL-10 to participate in the the immune allergic rhinitis machine purpose: to explore the peripheral blood of patients with AR and DE Th1 / T h2 cells function. Method: choose healthy controls HC 10, single house dust mite allergy in patients with perennial allergic rhinitis AR 10, desensitization efficacy of good patient to DE 8 people. Extracted from peripheral blood mononuclear cells the layer (PBMCs) were divided into a control group, to dust mites group and the PMA group, blank group joined the ionomycin 1ug/ml and monensin 2ug/ml; dust mite group joined ionomycin 1ug / ml and monensin 2ug/ml, dust mites stimulus becomes allergen of nDerp1 1 ug / ml; the PMA group joined the ionomycin 1ug/ml monensin 2ug/ml, PMA 20ng/ml after 37 ° C and cultured in a 5% CO2 incubator 6h. After PBMCs membrane and intracellular staining using flow cytometry detector test to determine of Th1 and Th2 in Tm (CD4 CD45RO T) cells, the percentage of research and analysis in peripheral blood of patients with AR and DE memory Th1 / Th2 cells function. Results: The percentage of blank group HC memory Th1 cells below AR, dust mites group HC group almost the same proportion of AR group increased, but not statistically significant; blank group HC DE memory Th2 AR group were higher than dust the mites after stimulation, HC, AR, and DE no significant changes, but the the Tcm Th2 cells in HC group was still higher than the AR group. PMA group, HC, AR and DE proportion of memory Th1 cells was significantly higher than the blank group (P lt; 0.05) Tcm Th2 cells was significantly lower than the control group (P lt; 0.05). Conclusion: AR house dust mite allergen stimulation immune-specific memory Th2 cells. PMA stimulation can promote memory Th cells secrete IFN-γ (memory Th1) and to inhibit memory Th cells secrete IL-4 (memory Th2).

Related Dissertations

  1. Effect of Grape Seed Procyanidins on the Blood Pressure in Renovascular Hypertensive Rats and Its Mechanism,R544.1
  2. Correlation of Humulus Pollen Allergic Rhinitis with HLA-DQA1 Gene,R765.21
  3. An Investigation of Allergens of Allergic Rhinitis and An Efficacy Analysis of 78 Cases Accepted Specific Immunotherapy in Dalian,R765.21
  4. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  5. The Expression and Clinical Significance of TH17 Cells Related Cytokines in the Patients with Rheumatoid Arthritis,R593.22
  6. Study on Mechanism of the Th17/Treg Imbalance in Toxoplasma Gondii Infected Pregnant Mice,R714.2
  7. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  8. Analysis on the Prevalence of Allergic Rhinitis among 3～ 7 Years Old Children in Urumqi , S First-grade Kindergarten,R765.21
  9. Septic lung injury (ALI / ARDS) in patients with peripheral immune parameters Th1, Th2, HLA-DR and Treg changes and Integrative Medicine,R459.7
  10. Bone marrow mesenchymal stem cells in the same kind of heart transplantation on Treg cells in rats,R654.2
  11. p38MAPK on allergic rhinitis peripheral blood mononuclear cells IL-4, IL-5 effects,R765.21
  12. Combined surgery 34 cases of severe persistent allergic rhinitis efficacy,R765.9
  13. Study on Expression of Th17 Cells and Treg Cells in Non-small Cell Lung Cancer in Elderly and the Relationship with Immunosenescence,R734.2
  14. The Dynamic Changes of Serum Cytokines in Paraquat Poisoning Rats and the Artesunate Intervention Studies,R595.4
  15. Study of Dendriti Cell Treatment Combined with Chemotherapy for Late Gastric Cancer,R735.2
  16. The Study of B7-H1/PD-1 Inhibitory Pathway and Relationship to Regulatory T Cells in Human Colon Carcinoma,R392
  17. The Th17/treg Regulation of Triptolide on Trinitrobenzene Sulfonic Acid-induced Colitis in Mouse,R285.5
  18. The Role of Fasudil in Treg Differentiation of Autoimmune Hepatitis Mice,R575.1
  19. Study of Atopy Patch Test in the Diagnosis of Allergy to Common Allergens in Atopic Dermatitis,R758.2
  20. A Preliminary Study on Serum Expression Level of IL-12 and IL-27 and Their Significances in the Patients with Allergic Rhinitis,R765.21

CLC: > Medicine, health > Otorhinolaryngology > Rhinology,nasal disease > Nasal disease > Rhinitis
© 2012 www.DissertationTopic.Net  Mobile