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Objective: To study the Guangxi Zhuang region, Han systemic scleroderma (systemic sclerosis, SSc) and HLA-DQA1,-DQB1 alleles. Methods: Using polymerase chain reaction - sequence specific primers (polymerase chain reaction-special sequence primers, PCR-SSP) method, the Guangxi Zhuang 50 cases, 50 patients with SSc Han and Zhuang, Han 100 healthy controls HLA-DQA1 10,-DQB1 5 allele typing. Results: HLA-DQA1 allele frequency distribution (1). Zhuang SSc patients group, eight alleles were detected, the gene frequency range of 0.000 to 0.300. DQA1 * 0102, * 0401 allele frequency was higher (0.210,0.300), DQA1 * 0103, * 0301 low frequency (0.040,0.050) were not detected, DQA1 * 0201, * 0302. 9 alleles were detected in the Zhuang the control group, the gene frequency range of 0.000 to 0.215. Which DQA1 * 0102 allele frequency was higher (0.215), DQA1 * 0103, * 0301 low frequency (0.050,0.055), DQA1 * 0302 were not detected. (2) Han SSc patients group, were found in eight allele gene frequency range of 0.000 to 0.190. DQA1 * 0101, * 0104, * 0601 allele frequency the (respectively 0.150,0.190,0.170), DQA1 * 0103, * 0301, * 0401 frequency lower (respectively 0.070,0.080,0.080) DQA1 * 0201, * 0302, were not detected. 9 alleles were detected in the Han control group, the gene frequency range of 0.000 to 0.225. Which DQA1 * 0101, * 0102 allele frequency was higher (0.200,0.225), DQA1 * 0103, * 0301, * 0601 frequency lower (respectively 0.060,0.070,0.030) were not detected, DQA1 * 0302 . 2. HLA-DQB1 allele frequency distribution (1). Zhuang SSc patients group, four alleles were detected in gene frequency range of 0.000 to 0.190. DQB1 * 0301, 0501, * 0601 allele frequency (respectively 0.150,0.120,0.190), low frequency of DQB1 * 0201 (0.080) and DQB1 * 0401 were not detected. The Zhuang the control group, four alleles were detected in gene frequency range of 0.000 to 0.140. DQB1 * 0301 allele frequency (0.140), DQB1 * 0501, * 0601 low frequency (0.040,0.075), DQB1 * 0401 were not detected. (2) Han SSc patients group, were found in four allele gene frequency range of 0.000 to 0.180. DQB1 * 0601 allele frequency (0.180), DQB1 * 0201, * 0501 low frequency (0.070,0.050), DQB1 * 0401 were not detected. The the Han control group, four alleles were detected, the gene frequency range of 0.000 to 0.135. DQB1 * 0201 allele frequency (0.135), lower frequency of DQB1 * 0601 (0.065) and DQB1 * 0401 were not detected. 3. HLA-DQA1, DQB1 allele frequency comparison (1). Zhuang SSc patients group and normal control group of HLA-DQA1-DQB1 allele frequency comparison, Zhuang SSc patient group in the HLA-DQA1 * 0401 - DQB1 * 0501,-DQB1 * 0601 gene frequency was significantly higher (0.300 VS 0.135, RR = 4.056, χ ~ 2 = 15.407, Pc = 0.001 and 0.120 VS 0.040, RR = 4.472, χ to 2 = 10.653, Pc = 0.004 and 0.190 VS 0.075, RR = 3.473, χ ~ 2 = 10.06, Pc = 0.008), there was significant difference between the two groups. (2) Han SSc patients group and normal control group of HLA-DQA1-DQB1 allele frequencies between Han SSc patients group HLA-DQA1 * 0401-DQA1 * 0601 - DQB1 * 0601 gene frequency significantly increased ( 0.080 VS 0.010, RR = 9.333, χ ~ 2 = 8.371, Pc = 0.036 and 0.170 VS 0.030, RR = 8.071, of χ ~~ 2 = 20.130, Pc = 0.000 and 0.180 VS 0.065, RR = 3.764, χ to 2 = 10.755, Pc = 0.004), there was significant difference between the two groups. (3). Zhuang, Han SSc patients group and normal control group allele frequencies of HLA-DQA1, Zhuang, HLA-DQA1 * 0201 gene frequency in Han Chinese patients with SSc patients group were significantly lower (0.000 VS 0.115, χ ~ 2 = 13.583, Pc = 0.002 and 0.000 VS 0.105 of χ ~~ 2 = 12.209, Pc = 0.004), the difference between the groups was statistically significant. (4) other allele groups showed no significant difference. Conclusion: HLA-DQA1 * 0401-DQB1 * 0601 may be a common susceptibility genes of Guangxi Zhuang, Han SSc patients, HLA-DQB1 * 0501 Guangxi Zhuang SSc patients susceptibility genes, HLA-DQA1 * 0601 Guangxi Han SSc patients susceptibility genes, HLA-DQA1 * 0201 may be protective genes of Guangxi Zhuang and Han SSc patients.
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