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Objective To observe the different concentrations of arsenic trioxide (Arsenic trioxide, AS203) dendritic cell differentiation and maturation and function of peripheral blood of patients with SLE in vitro culture, explore its mechanism, preliminary clarify dendritic cells (Dendritic cells.DCs) AS203 treatment the SLE role of targets. Isolated patients with SLE peripheral blood mononuclear cells (Peripheral blood mononuclear cells, PBMCs), granulocyte-macrophage colony-stimulating factor (granulocyte macrophagecolony stimulating factor, GM-CSF), interleukin -4 (interleukin-4, IL- 4) cytokine-induced DCs mature, adding different concentrations of AS203 and CTX culture morphology was observed under an inverted microscope. Collected on day 9 DCs cells, cell morphology was observed by scanning electron microscopy and transmission electron microscopy, CD80, CD86, and HLA-DR expression by flow cytometry. MTT assay DCs to stimulate lymphocyte proliferation ELISA assay mixed lymphocyte culture supernatant interleukin -10 (Interleukin-10, IL-10) and interferon-γ (Interferon-γ, IFN-γ ) levels. Results. Via AS2O3 and CTX treated DCs morphology in the immature state, AS203 and CTX, AS2O3 role more obvious; DCs express 2.AS203 and CTX treatment of CD80, CD86 and HLA-DR percentage compared with the control group was significantly lower, the difference was statistically significant (P lt; 0.05), and with the increasing concentration of DCs expression of CD80, CD86 and HLA-DR percentage decreased more significantly (P lt; 0.05); than AS203 CTX DCs role more obviously, the difference was statistically significant (P lt; 0.05). Mixed culture 3.AS203 and CTX treated DCs and T cells, stimulating T cell proliferation OD value significantly lower than the control group, the difference was statistically significant (P lt; 0.05), and with the increase in the concentration of reducing the more obvious ; AS203 role of DCs is more obvious, and the difference was statistically significant (P lt; 0.05). 4. Mixed culture supernatant IL-10 levels significantly lower than the control group, the difference was statistically significant (P lt; 0.05), while the level of IFN-γ was no statistical difference (P gt; 0.05); AS2O3 with CTX compared, the former inhibit the role of IL-10 secretion more significant, the difference was statistically significant (P lt; 0.05) Conclusion: The use of GM-CSF combined with IL-4-induced human peripheral blood mononuclear cells, can get high purity DCs; vitro culture conditions, AS2O3 can significantly inhibit DCs maturation and function of patients with SLE, and a dose-dependent inhibition the mature DCs stronger in CTX.
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