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The Role of Xuezhikang on Intima-media Thickness in Rats with Diabetes Mellitus

Author: ZuoGuangMin
Tutor: LiLi
School: Shanxi Medical
Course: Integrative Medicine
Keywords: Xuezhikang Diabetes Type 2 Macroangiopathy Tumor necrosis factor -α Carotid intima
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 25
Quote: 0
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Abstract


The purpose using animal experiments, the treatment of type 2 diabetes, high sugar high fat feeding induced rat serum and carotid artery tumor necrosis factor-α (TNF-α) expression changes observed Xuezhikang intervention simultaneous detection of the carotid artery before and after the intervention in film thickness (IMT), explore Xuezhikang improve atherosclerosis in type 2 diabetes. Method, 4-5 weeks of age, 54 (150g-180g) clean male Wistar rats were fed for one week, adaptability (5 / cage) were randomly divided into a control group (blank control group, group A, n = 18) and type 2 diabetes mellitus (T2DM group, n = 36), in which the control group received conventional feed (13.4kJ / g, which accounted for 10.2% of the fat to provide calories, protein accounted for 23.3%, 66.5% carbohydrate); Type 2 diabetes group given the high sugar high fat diet (21.8kJ / g, which accounted for 56.0% of the fat to provide calories, protein accounted for 7.0%, 37.0% carbohydrate). Free access to water at room temperature is controlled at 18 ~ 22 ℃, relative humidity of 30% to 70%. Two groups of rats daily morning and evening feeding twice, investment and food intake of each rat daily 3% of its weight. 12 weeks, do a glucose tolerance test (OGTT): 8h After fasting tail blood fast blood glucose meter for measuring fasting blood glucose (FBG), 50% glucose injection 2g/kg body weight orally again after 2 hours rat tail blood measured 2h postprandial blood glucose (2hPG). ≥ 16.7mmol / L and 2-hour postprandial the glycemic (2hPG) modeling success for more than a week. Control group, normal blood sugar, and continue to give regular diet, with free access to water; Type 2 diabetic model rats were randomly divided into a control group of diabetes (T2DM control group, group B, n = 18), Xuezhikang intervention group (T2DM intervention group, C The group, n = 18), both groups continue to give high sugar high fat feeding, free drinking water. The of T2DM control group and T2DM intervention group continued for 12 weeks after the start Xuezhikang intervention. The Xuezhikang intervention group was given the Xuezhikang 0.15g/kg/d (7.5 times the human dosage) suspension orally, blank control group and T2DM control group received an equal volume of saline. In the first four weeks, eight weeks, 12 weeks from each of six Wistar rats weighing fasting one day before the end of the experiment, 12 ~ 14h, tail blood fast blood glucose meter for measuring fasting glucose, followed by 5% the chloral hydrate 0.6ml/100g body weight intraperitoneal injection of anesthetized rats, abdominal vein, blood, blood samples were rapidly centrifuged specimens from serum -70 ℃ save analyte relevant indicators. Part of the cut carotid artery tissue frozen at -70 ℃ refrigerator for RT-PCR assay the expression of TNF-α, another part of the fixed embedding prepared biopsy for light microscopy carotid artery morphologic changes and determination medial thickness in carotid IMT. SPSS13.0 statistical software for data applications, the results of the measurement data are presented as mean ± standard deviation (x ± s) said; completely randomized factorial design analysis of variance; correlation using Pearson correlation analysis, taking P lt; 0.05 was considered statistically significant. The 1.B group and group C serum levels of TNF-α level compared with group A significant rise, the difference was statistically significant (P lt; 0.05); reduce the C group than in the group B serum TNF-α levels, the difference was statistically significance (P lt; 0.01). 2. RT-PCR results show that TNF-α mRNA expression in rat carotid arterial tissue in group B and group C than in group A was significantly higher, the difference was statistically significant (P lt; 0.01); C group and B group TNF-α mRNA expression in the carotid artery tissue decreased, and the difference was statistically significant (P lt; 0.01). 3. Carotid intima media thickness IMT: group B and group C than in group A was significantly increased, the difference was statistically significant (P lt; 0.01); reduce group than in the C group B, the difference was statistically significant (P lt; 0.01). Light microscope observation organization morphological changes: B rats carotid artery endothelial cells arranged gradually disorder, obvious proliferation of medial smooth muscle cells, resulting in the carotid arterial wall thickening, stenosis. After after Xuezhikang intervention, the pathological changes significantly reduced, and the lesions improved more significantly over time. 5 linear correlation analysis showed that: the presence of significantly positive correlation between the type 2 diabetic rat carotid artery TNF-α mRNA expression IMT With the TNF-αmRNA carotid express the amount of the increase, IMT gradually increasing. Conclusion TNF-α expression in the serum, carotid artery tissue levels associated with type 2 diabetic macroangiopathy the whole process, and gradually increased with the duration of the development. Xuezhikang intervention significantly reduced carotid IMT the histomorphological improved, the diabetic macroangiopathy gradually reduce. Correlation analysis showed a positive correlation between the expression of TNF-α in carotid and carotid IMT. Mechanisms the Xuezhikang reduce carotid IMT by down-regulating the expression of TNF-α. Speculated Xuezhikang vascular protective effects other than lipid-lowering.

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