Dissertation > Excellent graduate degree dissertation topics show

The Effects of Dynamic Compressive Stress and PTHrP on the Biological Characteristics of Rat Precartilaginous Stem Cells in Vitro

Author: ChenJie
Tutor: ChenAnMin
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: PSCs dynamic stress stimulation PTHrP Sox9 Proliferation extracellular matrix
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 2
Quote: 0
Read: Download Dissertation

Abstract


Objective Culture precartilagious stem cells planar and in calcium alginate beads, add the dynamic stress stimulation or/and thyroid hormone related peptide (PTHrP) to different groups, further revealed the proliferation and differentiation rule of PSCs and regulation mechanism.Methods The PSCs in femur epiphysis of new SD rats were isolated and purified using magnetic activated cell sorting system and cultured in vitro planar and in calcium alginate beads. Cells were randomly divided into different groups and subjected to PTHrP of different concentrations or/and compressive pressure of 90 mmHg. Cell proliferation be detected through Cell count and Cell Counting Kit-8(cck8). The specificity extracellular matrix - typeⅡCollagen of 3d cultivate cartilage cells be detected through immunohistochemical .The expression levels of typeⅡcollagen,type X collagen, aggrecan,Sox9, IGF-1, TGF-β1and PTHrP were measured by reverse transcription polymerase chain reaction(RT—PCR).Results Good state of PSCs are separated from mixture of cells throu relatively tumor-specific markers with PCNA ne fluorescence, it is displayed that cells expression FGFR - 3 which is relative specific markers and PCNA which is relatively tumor-specific markers; The cell proliferation capacity be detected though cell count and CCK - 8 method, The results show that the effective intervention group increase obviously than control group (P﹤0.05) and PTHrP intervention and stress stimulus have a synergy effect. RT - PCR tests showed that in effective intervention groups the expression of CollagenⅡ, aggrecan, Sox9 is elevatory than the control group, Collagen X expression is reduced than the control group (P﹤0.05), that the expression level of PTHrP, TGF - beta 1, IGF– 1 in 90mmg pressure stimulate group is higher than the control group (P﹤0.05) .Conclusions In vitro both PTHrP intervention and compressive stress stimulus can affect the proliferation and differentiation of PSCs;In 90mmg pressure intermittent stimulate group, the Proliferation effect is evident; The terminal differentiation of cells is delayed; Maybe it is relevant to the elevatory expression level of PTHrP; The 0.1nmol /L PTHrP have a Stimulative effect to the proliferation of PSCs and a delay effect on its terminal differentiation; Sox9 is its probable targets; the dynamic stress stimulation and thyroid hormone related peptide have a synergetic effect on promoting the proliferation and extracellular matrix secretion.

Related Dissertations

  1. The Effect of Low-intensity Pulsed Ultrasound on Cell Proliferation and the mRNA Expression of Extracellular Matrix of SD Rat Precartilagious Stem Cells,R329
  2. Bi Shu lumbar intervertebral disc degeneration of rabbit IL-1α mRNA expression levels and the impact of Sox9,R285.5
  3. Preparation and Identification of Recombinant Adenoviruses Carrying Short Hairpin Rna Targeting PTHrP of Goat,S852.65
  4. Comparative Studies about the Expression of Vg, Sox9, Sox9a2, Dmrt1 Gene in Diploid and Triploid Female Rainbow Trout (Oncorhynchus Mykiss),S917.4
  5. Effect of Induced Occlusal Disorders on the Expression of Sox9, PTHrP and PTH1R of Condylar Cartilage in Rats,R783
  6. Systematic Review of Tacrolimus Versus CsA-ME in the Efficacy, Safety and Cost-Effective Analysis of SPK Recipients, and Study of Their Effect on the PSCs in Fibrosis,R617
  7. Bovine Sry Core Promoter Localization and Gene Functional Characterization,S823
  8. Study on Sex Related Genes Sox9, Ftz-F1, and P450arom in Yellow Catfish, Pelteobagrus Fulvidraco,S917.4
  9. Effect of MCP-1/CCR2 Axis on Prostate Cancer Skeletal Metastasis,R737.25
  10. Sublytic C5b-9 induced GMCs proliferation and ECM secretion molecular mechanisms and signaling pathways of,R692.3
  11. Cloning, Prokaryotic Expression of Goat PTHrP, and Its Antibody Preparation,S827
  12. Effect of Noredrenaline and Phentolamine in the Hepatic Stellate Cells of Rat,R575.2
  13. Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-like Cells by Transfected with SOX9 Eukaryotic Expression Vector in Vitro,R681.53
  14. A Fundamental Research into the Biological Regulation of Human Parathyroid Hormone1-34 and Hypotonic Solution on Osteoblast-like Cell MG63,R580
  15. Effects of HMG-CoA Reductase Inhibitor on Proliferation and Extracellular Matrix Secretion in Cyst-Lining Epithelial Cells and Fibroblasts of Autosomal Dominant Polycystic Kidney Disease,R692.1
  16. Experimental Study of Chondrogenesis Differentiation of MSCs in Vitro and in Vivo,R329.2
  17. The Study on Dynamic Changes of Apoptosis and Proliferation of Lung Cells, Interstitial Fibrosis and Their Mechanisms in Premature Rats with Hyperoxia-induced CLD,R722.6
  18. Curcumin proliferative glomerulonephritis and experimental study of the preventive effect and its mechanism to explore,R692.3
  19. Construction of Immortalized Precartilaginous Stem Cells and Experimental Study on Sox9’s Inductive Effects on Directed Differentiation of Bone Marrow Stromal Cells,R329
  20. ADAMTS-7 and ADAMTS-12: Novel Mediators of Chondrogenesis,R341

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile