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[Objective] by detecting myeloproliferative syndrome (MDS), the bone marrow dysplasia, type and quantity of analysis in MDS mainly what type of dysplasia, a quantitative analysis of the development of abnormal cells in order to investigate the type and number of developmental abnormalities MDS diagnosis, classification, prognosis whether certain clinical significance. 【Methods】 Union Medical College Hospital from January 2005 to January 2011, 145 cases of MDS (according to WHO classification criteria), including refractory anemia (RA), refractory anemia with ring sideroblasts cells increased (RARS), hard the treatment of anemia associated with multi-lineage developmental abnormalities (RCMD), refractory anemia with excess blasts I (RAEB I), refractory anemia with excess blasts type Ⅱ (RAEB II) 5q-syndrome patients clinical data and laboratory Check the results. Bone marrow samples of these patients with Switzerland staining in oil microscope, were counted one hundred non-primitive neutrophils, erythrocytes and fifty megakaryocytic dysplasia cell number using Excel software to create detailed data files, SPSS15.0 software package was used for statistical analysis of the results of the relevant data. While other diseases can appear dysplasia cells [1] such as the decrease in megaloblastic anemia (MA), idiopathic thrombocytopenic purpura (ITP). Chronic myeloid leukemia (CML), and systemic lupus positions (SLE) 7 cases were compared. [Results】 MDS bone marrow dysplasia is easy to see, faculties dysplasia can be seen in other diseases. The developmental abnormalities in MDS and non-MDS compared the faculties dysplasia percentage and faculties types of developmental abnormalities differences than other diseases, most of the non-the MDS patients faculties dysplasia percentage did not exceed 10%; and some developmental abnormalities (such as PH deformity lymphoid megakaryocytes) detection rate and mean than MDS. Therefore, quantitative analysis of MDS dysplasia the MDS diagnosis has important significance. MDS subtypes, karyotype, IPSS group comparison: reduced cytoplasmic granules, nuclear pulp developmental imbalance, PH deformity and lymphoid small megakaryocytes detection rate and the mean was statistically significant (P <0.05), in RAEB, 7q- / complex karyotype group, the highest of the high-risk group, the detection rate of 100%. Prompt decrease in cytoplasmic granules, nuclear pulp developmental imbalance, PH deformity, the lymphoid the small giant check the MDS prognosis influential MDS adverse prognostic factors. Erythroid developmental abnormalities compared various subtypes, karyotype, IPSS grouping mean detection rate without statistical significance (P gt; 0.05), suggesting that abnormal erythroid development had no effect on the prognosis of MDS. [Conclusion] a quantitative analysis of developmental abnormalities have important significance for the diagnosis of MDS, can provide the basis for the differential diagnosis of MDS and non-MDS: MDS, most of the non-MDS patients faculties dysplasia does not exceed 10%; Some dysplasia ( PH deformity and lymphoid megakaryocytes) in MDS patients mean detection rate compared with non-MDS. 2 dysplasia type MDS prognosis: nuclear pulp developmental imbalance, reduced cytoplasmic granules, PH deformity lymphoid megakaryocytic adverse prognostic factors of MDS.
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