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Steroids endothelial growth factor -1 (SF-1) is one of the orphan nuclear receptor superfamily . Originally isolated from the adrenal gland cDNA library were expressed in the major steroidogenic tissue , pituitary and hypothalamic ventromedial nucleus . It plays an important role in steroidogenesis , adrenal and gonadal development and sex differentiation of physiological processes , the main regulatory factor is a the Human Cytochrome protein P450 (Cyp19a1) expression . Many studies have shown that the SF-1 interaction with a variety of proteins , however , there are some not yet discovered , factors involved in the SF-1 on different target genes . First, we studied the TGF-β3 by SF-1 mediated estradiol ( e 2 < / sub >) is generated, found SF-1 , a binding protein of the TGF -beta superfamily members Smad3 . Through immunization co-precipitation experiments confirmed that SF-1 interacts with Smad3 and SF-1 regulation provides new clues for us to explore . Secondly , we study the significance of SF-1 combined with Smad3 , SF-1 and Smad3 synergistic estradiol to generate the rate-limiting enzyme in of Cyp19a1 the mRNA expression . In addition , SF-1 and Smad3 are able Cyp19a1 P Ⅱ promoter combine and influence each other. Therefore , Smad3 combine jointly promote the generation of estradiol as a crossover point SF-1 and TGF signaling pathway . This provides a basis for further understanding of the regulation of SF-1 function . Finally, we study the TGF-β3 to promote the growth of mouse follicle , SF-1 to participate and play a role in granulosa cell proliferation process . SF-1 function in cell proliferation has at home and abroad has not been reported , and these results to a more in-depth exploration of the role of SF-1 provides new clues . In conclusion , our study found that the SF-1 , a new co - activating factor the Smad3, revealing their interactions in estrogen production , and the two signaling pathways combined . Understand the impact of potential extracellular signals generated by estrogen crosspoint help identify therapeutic targets of estrogen -related diseases .
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