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The Changes of Regulatory T Cells in Different Periods of COPD

Author: DengLi
Tutor: XiongXianZhi
School: Huazhong University of Science and Technology
Course: Pulmonology
Keywords: Regulatory T cells Chronic obstructive pulmonary disease Cytokines Tiotropium
CLC: R563.9
Type: Master's thesis
Year: 2011
Downloads: 30
Quote: 0
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Abstract


Objective To investigate the changes of regulatory T cells in different stages of chronic obstructive pulmonary disease (COPD), to investigate acute exacerbation of COPD midterm cytokine changes. Method to extract the normal control group of 20 cases, 21 cases of acute exacerbation of COPD peripheral venous blood before and after treatment of patients and 23 patients with stable COPD patients after tiotropium, the use of three-color-labeled monoclonal antibody flow cytometry, detection of peripheral blood T cell subsets CD 4 , CD 4 CD 2 5 , CD 4 CD 2 5 FoxP3 T (CD < sub> 4 Treg), CD 8 , CD 8 CD 2 5 , CD 8 CD 2 5 of FoxP3 (the CD 8 Treg) percentages and numbers. TGF-β in the peripheral blood of acute exacerbation of COPD measured by enzyme-linked immunosorbent assay (ELISA), TNF-α, IL-9, IL-10 and IL-17 concentration. Results in peripheral blood CD / sub> . the T cells and CD of 8 T cells in the acute phase of COPD, with stable and normal between the control group had no significant difference (F = 0.74, P = -0.48); CD 4 CD 2 < / sup> T cells in acute exacerbation of COPD, with stable and normal control group were significant differences (F = 33.08, P lt; 0.01), and in the acute phase of COPD aggravated interim number of the most significant; CD 4 Treg percentage in acute exacerbation of COPD phase and stationary phase was significantly decreased compared with normal control group, but CD to 4 / sub> a the number of Treg cells in acute exacerbation of COPD COPD compared with stable and normal control group significantly increased in CD 4 / sub> . Treg percentage and the number of stable COPD and normal The control group did not significantly difference; of the CD 4 Treg total percentage of lymphocytes as well as accounting for the CD 4 T cells percentage in the acute phase of COPD than those of the other two groups significantly increased; compared with stable COPD and normal control group, CD 8 CD 2 the Treg percentage of 5 T cells in acute exacerbation of COPD increased significantly the the CD 8 / sub> . in acute exacerbation of COPD and stable period compared to the normal control group expression was significantly decreased, but the the CD 8 of Treg cell numbers in acute exacerbation of COPD significantly increased relative expression of stable COPD, with no significant difference between the normal control group ; CD 8 . proportion accounted Treg lymphocytes in COPD acute phase exacerbation of more stable period was significantly higher; Treg number of acute exacerbation of COPD and Treg accounted lymphocytes proportion than in stable COPD and normal control group significantly increased. The treatment of stable COPD by tiotropium CD 4 the CD the 2 T cells in the expression level of decreased significantly, almost reached the level of the normal control group, the CD 8 Treg occurs significantly improved. Acute exacerbation of COPD midterm TGF-β, TNF-α, IL-9, IL-10 and IL-17 concentration increased compared with the normal level of the control group. Patients with acute exacerbation of COPD, CD 4 Treg and leukocytes, neutrophils, a negative correlation was positively correlated with lymphocyte; CD 8 Treg smoking index was negatively correlated; IL-10 and smoking index was negatively correlated, and white blood cells was positively correlated; IL-9 and IL-10 was positively correlated with IL-17 showed a negative correlation. Conclusion regulatory T cells in the pathogenesis of COPD play an important role, due to a variety of pathogenic factor makes regulatory T cells in response to low enough to suppress excessive inflammatory response, making COPD delayed healing. Muscarinic receptor has a complex impact on the regulatory T cells, cytokine has an important role in the pathogenesis of COPD.

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