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Study of Upregulation of Matrix Metalloproteinase-2 Expression Mediated by ERK1/2/MAPK Signaling Pathway Promoting Drug Resistance in Osteosarcoma

Author: DengZuo
Tutor: ChenAnMin
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: Osteosarcoma ERK1 / 2 MMP-2 Resistance
CLC: R738.1
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 0
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Abstract


The purpose confirmed ERK1/2/MAPK pathway mediated regulation of MMP-2 induced osteosarcoma resistant , and provide the basis of its drug molecular targeted therapy . The gradient concentration ADM shock induction method to develop osteosarcoma MG-63 cells induced to build a drug-resistant cell lines MG-63 , and blocking ERK1/2/MAPK pathways , cell proliferation cck-8 assay , growth curve ; blocked before and after the expression of MMP-2 mRNA was detected by RT-PCR ; the ADM role before induction and after blocking MG-63 cells using Annexin V / PI double staining to detect the apoptosis rate . Results of RT-PCR reveals resistant MG-63 cell line , MMP-2 mRNA expression was significantly increased than normal cell lines , Annexin V / PI double staining prompted resistance by ADM induced MG-63 cell lines and blocking MG -63 / RI cell apoptosis rate than before the induction of cells decreased significantly. The conclusion ERK1/2/MAPK pathway mediated MMP-2 upregulation , leading to the emergence of resistance of MG-63 cells .

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CLC: > Medicine, health > Oncology > Sports Department tumor > Bone tumor
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