Dissertation > Excellent graduate degree dissertation topics show

Expression of CR16 in Testicular Tissue and the Initial Exploration of Its Role in Pathogenesis of Azoospermia

Author: WenZiNa
Tutor: XiangWenPei
School: Huazhong University of Science and Technology
Course: Obstetrics and Gynaecology
Keywords: Corticosteroids and regional expression 16 Sprague-Dwaley rats testes Sertoli cells Sertoli cell-spermatid junctions non-obstructive azoospermia obstructive azoospermia neural Wiscott-Aldrich syndrome protein azoospermia N-WASP-CR16 protein complex
CLC: R698
Type: Master's thesis
Year: 2010
Downloads: 8
Quote: 0
Read: Download Dissertation

Abstract


PartⅠThe expression of CR16 in testicular tissue of normal fertile Sprague-Dwaley ratsObjective To investigate the expression of Corticosteroids and regional expression 16(CR16)in testicular tissue of normal fertile Sprague-Dwaley(SD)rats and provide an initial animal experiments and methods preparation for further study of CR16 in the human reproductive system.Methods Immunohistochemical was used to measure the levels of CR16 protein in the testes of 5 normal fertile SD rats.Results Immunohistochemical showed that CR16 protein expressed in the Sertoli cells and Sertoli cell-spermatid junction(sSspJs)in epithelial of seminiferous tubules.Conclusion The CR16 protein expressed in the Sertoli cells and SspJs in testicular tissue of normal fertile SD rats, which indicates that CR16 may have relationship with spermatogenesis. PartⅡThe expression of CR16 in testicular tissue of azoospermia patients and healthy menObjective To investigate the expression of CR16 in the testes of patients with obstructive azoospermia' dissertation">non-obstructive azoospermia, obstructive azoospermia and healthy men and discuss its correlation with azoospermia.Methods Immunohistochemical and reverse transcript polymerase chain reaction (RT-PCR) were used to measure the levels of CR16 protein and mRNA in the testes of 6 patients with non-obstructive azoospermia, 23 patients with obstructive azoospermia and 6 healthy men.Results Immunohistochemical showed that CR16 protein expressed in the cytoplasm and membrane of Sertoli cells and SspJs in epithelial of seminiferous tubules. And the level of CR16 protein was markedly lower in non-obstructive azoospermia patients than obstructive azoospermia patients and healthy men. RT-PCR showed that the level of CR16 mRNA was significantly decreased in non-obstructive azoospermia patients(0.640±0.021)compared with the healthy men(0.952±0.036)(P<0.01), and had no statistical significance between obstructive azoospermia patients(0.953±0.036)and healthy men(P>0.05).Conclusion The levels of CR16 protein and mRNA decrease in the testes of non-obstructive azoospermia patients, which indicates that CR16 may have relationship with pathogenesis of azoospermia and participate in spermatogenesis dysfunction. Objective To determine the expression of neural Wiscott-Aldrich syndrome protein (N-WASP) in the testes of patients with non-obstructive azoospermia and healthy men and discuss its correlation with CR16 in humen testes and their role in spermatogenesis.Method Immunohistochemical and RT-PCR were used to measure the levels of N-WASP protein and mRNA in the testes of 6 patients with non-obstructive azoospermia and 6 healthy men. Double-staining immunofluorescence and laser scanning confocal microscopy (LSCM) were used to demonstrate co-localization of CR16 and N-WASP proteins.Results Immunohistochemical showed that N-WASP protein expressed in the cytoplasm and membrane of Sertoli cells and SspJs in epithelial of seminiferous tubules. And the level of N-WASP protein was markedly lower in non-obstructive azoospermia patients than healthy men. RT-PCR showed that the level of N-WASP mRNA was significantly decreased in non-obstructive azoospermia patients(0.645±0.025)compared with the healthy men(0.960±0.031)(P<0.01). The ratio of CR16 and N-WASP mRNA was approximately 1:1 in both groups of men. The distribution of CR16 and N-WASP was similar in the epithelium of seminiferous tubules based on double-staining immunofluorescence and LSCM, thereby suggesting that CR16 and N-WASP were co-localized in the SspJs as a N-WASP-CR16 protein complex.Conclusion The decreased levels of CR16 and N-WASP in the testes of men with non-obstructive azoospermia compared with healthy men and the co-localization at SspJs as a N-WASP-CR16 protein complex in the epithelium of seminiferous tubules suggests a role in the pathogenesis of non-obstructive azoospermia.

Related Dissertations

  1. Analysis of Chromosome Abnormalities and AZF Microdeletions in Infertile Men,R698.2
  2. Study on the Different Expression of Heme Oxygenase Enzyme in Human Testis of Fertile and Infertile Males,R698.2
  3. The Preliminary Study of PK2 Expression in Human Testis and Its Potential Funtion in Spermiogenesis,R698.2
  4. Study on Association between Single Nucleotide Polymorphisms of PATZ1 Gene as Well as DNMT3L Gene and Azoospermia,R698.2
  5. To Analysis the Diagnosis and Treatment of 89 Patients with Azoospermia,R698.2
  6. Bcl-2 coding region polymorphisms and male spermatogenesis and mechanism of the correlation,R698.2
  7. Genetic Analysis on Infertile Male Patients,R698.2
  8. Low dose of dibutyl phthalate (DBP) in male rats of the reproductive system interference,R114
  9. The Study of Microsurgical Longitudinal 2-stuture Intussusceptive Vasoepididymostomy for the Treatment of Epididymal Obstructive Azoospermia.,R698.2
  10. The Initial Exploration of Endogenous Ouabain in the Pathogenesis of Asthenospermia,R698.2
  11. Screening for Microdeletions of AZF Region on the Y Chromosome and Point Mutations on DAZ Gene in Infertile Men,R698.2
  12. Effects of P, P’-DDE and β-BHC in the Expression ofCalcium、erk and BCL-2 in Rat Sertoli Cells,R114
  13. The Effects of Lead Acetate on the Expression of Androgen Binding Protein, Transferrin and Inhibin mRNA in Rat Sertoli Cells,R114
  14. Effects of Energy-protein Levels on Reproduction Performancein Late Reproductive Period of Beijing-you Chickens and Construction of Gene-expression Profiles in the Testicular Azoosperma,S831.3
  15. The Expression Levels of Clusterin in the Testis of Infertility Men,R698.2
  16. Study on the Mechanism of the Carbendazim-induced Reproductive Toxicity in Male Rats,R114
  17. The Apoptosis Effects of Microcystin-LR on Rats Sertoli Cells,R114
  18. The Effect of MnCl2 on the mRNA Transcription and Protein Expression of Androgen Binding Protein, Transferrin and Inhibin B in Rat Sertoli Cell,R114
  19. Proteomics Study of Di-n-butyl Phthalate Damagment to Rat Sertoli Cells,R114
  20. Research on the Toxicity Mechanism of Solanine on Cells of Mice Testis,R285

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Men 's sexual dysfunction
© 2012 www.DissertationTopic.Net  Mobile