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Pigskin collagen extraction and modification of succinylated

Author: LiXingWu
Tutor: LiHongJun
School: Southwestern University
Course: Of Food Science
Keywords: Pigskin Microwave treatment Collagen Succinylation Features
CLC: TS251.92
Type: Master's thesis
Year: 2011
Downloads: 321
Quote: 2
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Abstract

Because collagen has a unique amino acid composition and structure, is widely used in medical, health and beauty, and other fields. China is the world one of the largest pig production and pig skin collagen content rich resource development pigskin, pig processing on the beneficial recycling of waste, turning waste into treasure has important practical significance. This topic aims at pigskin as raw material, the use of microwave treatment to improve the extraction rate of collagen, and through succinic anhydride molecular collagen extracted modified to improve its functional properties, expand its applications. In this paper, Rongchang pig pigskin selected as a raw material in the various components of the pigskin content; pigskin degreasing agent selection; microwave pretreatment and extraction technology on the pigskin collagen extraction rate; extracted collagen Identification; succinylated collagen modification; acylated collagen property measurement. The main results are summarized as follows: (1) Determination of moisture content obtained in pig Rongchang 39.92%, crude fat content of 33.74%, 2.13% hydroxyproline content, crude protein content of 26.46%, of which collagen protein accounted for 80 %. And the analysis and comparison of the effect of different degreasing degreasing agent, degreasing effect obtained chloroform: ethanol (2:1; V: V) gt; 5% Na2CO3 gt; 1% lipase gt; ether: petroleum ether (1:1; V: V) gt; petroleum ether gt; ether. (2) The effects of different factors on the extraction rate of collagen, and the extraction rate target parameters, respectively, of the microwave treatment temperature, microwave processing time, solid-liquid ratio and extraction time four orthogonal factors. The results showed that four factors influence collagen extraction rate of primary and secondary order solid-liquid ratio gt; extraction time gt; microwave time gt; microwave temperatures. Optimal level of microwave time 2min, microwave temperature 25 ℃, solid-liquid ratio 1:30, extraction time 35h. (3) before and after microwave treatment produced pigskin paraffin sections, comparative analysis of results found by microwave treatment pigskin collagen fibers toward clearer, shorter and thicker portion of the collagen fibers and collagen fibers are dispersed, indicating that the microwave on collagen fibers a strong dispersion. Pigskin after microwave treatment after extraction rate can achieve higher yields. (4) Identification of collagen mentioned maximum absorption wavelength of the UV absorption at 235 nm wavelength, in full compliance with the characteristics of collagen; infrared spectroscopy after microwave treatment collagen extracted sample with a more complete three-helix structure, the basic retain the natural collagen shape; electrophoretic analysis of the three collagen peptide chain molecular weight greater than 97kDa, and collagen Ⅰ similar molecular weight. Collagen denaturation temperature measurement reflects collagen extracted with good space structure and biological activity. (5) The collagen with succinic anhydride modified, can be obtained by IR spectroscopy of collagen succinic anhydride to introduce a hydrophilic group. Single factor test results added succinic anhydride, reaction time, reaction temperature, pH value of the succinylated collagen have a significant impact on the modified. According to the results of single factor test, response surface design experiment. Analysis results for succinylated extent the effect is the reaction time gt; anhydride dosage gt; temperature gt; pH. And acylated collagen with optimized to obtain the best conditions for the degree, the amount of succinic anhydride, 15.15%, pH value of 9.10, temperature 39 ℃, time 3.01h, the predicted time of collagen 71.446% theoretical value. (6) Determination of the nature of the collagen after acylation derived: When the collagen into succinic anhydride and an isoelectric point in the acidic environment of the offset, the greater the degree acylation isoelectric point is smaller; acylation collagen solubility significantly enhanced, higher degree of acylation generally higher solubility; acylated collagen emulsifying activity is enhanced, improving the emulsifying activity, expanding its scope of application; acylating for different levels of collagen, more acylation degree Large changes greater emulsion stability, but on the whole is greater than the stability of acylated collagen is not acylated collagen; acylation collagen gel strength than the acylation was significantly increased, but the maximum gel strength being biased shift to a lower pH value around. However acylation collagen until the temperature reaches 60 ℃, gel strength increases with temperature, when 60 ℃ ~ 80 ℃ no significant change in the gel strength, 80 ℃ after acylated collagen gel strength began to decline, and the greater the degree of acylation, the decrease increases.

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