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【Objective】The aim of this work was to explore the inhibitory effects of Corilagin on the production of pro-inflammatory cytokines in microglia stimulated by radiation.【Methods】The cytotoxicity of Corilagin was measured by MTT assay. Microglia BV-2 cells were stimulated by radiation(0, 32Gy)after pretreated with Corilagin for 12 hours. Realtime-PCR was used to detect the inflammatory cytokines such as IL-1β, TNF-αwhich were expressed by radiation-stimulated microglia BV cells on several time point. The production of Nitric Oxide (NO) was determined with nitrate reductase. The translocation of NF-κB p65 was measured by western-blot and immunocyto- chemical stain. Confocal microscopy was used to detected the expression of Iba-1, Nemo , IκB and the translocation of NF-κB p65.【Results】Corilagin showed no cytotoxicity for BV-2 cells within 1-10μg/mL. Irradiation was able to activate microglia cell with expression of Iba-1, and elevated the expression of inflammatory cytokines such as IL-1β, TNF-αand NO. Whereas, 5μg/mL Corilagin was significantly able to inhibit the production of IL-1β, TNF-αin activated microglia cells (tIL-1β=6.341,p<0.001;tTNF-α=3.411,p<0.007;tNO=3.134,p<0.015). Given a 32Gy of irradiation, NF-κB p65 in BV-2 translocate from cytoplasm to nuclear, correspondence with a increased Nemo and a inhibition of IκB, Corilagin significantly inhibited the expression of Nemo and the translocation and activation of NF-κB p65.【Conclusions】Corilagin could inhibit the activation of radiated microglia cells and down-regulate the expression of inflammatory cytokines, via inhibition of NF-κB signaling pathway.
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