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Study of the Regulation of TERE1/UBIAD1 in Cellular Proliferation

Author: WeiXiong
Tutor: HongLing
School: Huazhong University of Science and Technology
Course: Biomedical Engineering
Keywords: TERE1/UBIAD1 Isopentenyl transferase Ras-MAPK Cell proliferation
CLC: R730.2
Type: Master's thesis
Year: 2011
Downloads: 5
Quote: 0
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Abstract


Transitional epithelial reaction the gene ( Transitional epithelial response gene 1 TERE1 ) , located on chromosome 1p36 , encodes a 338 amino acid The molecular weight for 36.8KD protein widely expressed in a variety of human tissues , including urinary tract epithelial tissue . Its the containing UBiA class prenyltransferase enzyme function domain , so it is called UBIAD1 (UbiA prenyltransferase containing 1). Found in patients with bladder cancer and prostate cancer , TERE1/UBIAD1 protein expression was reduced; these two cancer cell lines proliferation rate was significantly lower in bladder cancer and prostate cancer cell lines overexpressing TERE1/UBIAD1 protein found . But did not clarify the mechanism of action in the inhibition of cell proliferation and TERE1/UBIAD1 . We found that the Ras - MAPK signaling pathway is active , TERE1/UBIAD1 protein expression in bladder cancer cell line T24 down . When the low TERE1/UBIAD1 knock in the fetal liver cell line L02 , Ras-MAPK signaling pathway is also activated , and determination of the cell cycle , and found an increase in the proportion of cells in S phase . Speculated TERE1/UBIAD1 protein through the Ras-MAPK signaling pathways to regulate cell proliferation process . TERE1/UBIAD1 prenyltransferase activity, whereas the the Ras protein post-translational modification process including prenylation . With direct interaction immunoprecipitation experiments confirmed TERE1/UBIAD1 with HRas . When the amount of protein knockdown human embryonic kidney cell line HEK293 TERE1/UBIAD1 found that the distribution of the BFP-HRas Golgi reduce , the use of mass spectrometry the analysis HRas enzymatic fragment found HRas farnesylation increased . In view of this , we speculate , may TERE1/UBIAD1 by farnesyl transferase out on HRas so HRas stranded in the Golgi functions to promote cell proliferation , not to the cell membrane , thereby regulating cell proliferation .

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CLC: > Medicine, health > Oncology > General issues > Tumor pathology, etiology
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