Dissertation > Excellent graduate degree dissertation topics show

IL-17A in experimental pulmonary fibrosis in mice tissues and its significance

Author: FuLiZhu
Tutor: LiQiZuo
School: Kunming Medical College
Course: Internal Medicine
Keywords: Th17 cells IL-17A Bleomycin Pulmonary fibrosis Dexamethasone Hydroxyproline
CLC: R563.9
Type: Master's thesis
Year: 2011
Downloads: 71
Quote: 0
Read: Download Dissertation

Abstract


[ Objective ] by observing Thl7 cells produce IL-17A in bleomycin-induced pulmonary fibrosis in mice expressing each period during glucocorticoid treatment and its expression after the changes of IL-17A in pulmonary fibrosis pathogenesis of the role and significance . [ Methods ] 1 would be 72 weighing 18-22g of C57BL/6J male mice were randomly divided into control group (NS group ) , the experimental group (BLM group ) and treatment group (DM group ) , each 24 ; 2.NS disposable endotracheal saline group (0.07 ~ 0.09ml), BLM group and DM group disposable endotracheal bleomycin injection of saline around 0.08ml (5mg/kg), to copy pulmonary fibrosis model , DM group after reperfusion in bleomycin 5mg/kg daily intraperitoneal injection of dexamethasone ; 3 . then each group were 7, 14 and 28 days of the first mice were sacrificed 6 , aseptic remove lung tissue pathology observed phases pulmonary inflammation and fibrosis , the determination of lung tissue hydroxyproline content , immunohistochemical staining the expression of IL-17A , RT-PCR detection of mRNA of IL-17A expression . [ Results ] 1 . Lung histopathology Tip : BLM group and DM group in the early stages of inflammation was no significant difference , while the latter BLM group of lung fibrosis was significantly higher than DM group and NS group (P lt; 0.05), NS group had no signs of inflammation and pulmonary fibrosis ; 2.BLM mice lung hydroxyproline content than NS group and DM group (P lt; 0.05); 3. immunohistochemical detection of IL-17A show mainly in the BLM group 7 and 14 days was significantly , the first 28 days has decreased , DM group was lighter but still higher (P lt; 0.05); 4.BLM group at day 7,14,28 IL -17AmRNA was significantly higher than the NS group and DM group (P lt; 0.05), which peaked at 14 days . DM group was lower than that of IL-17A BLM group , compared with NS group is still statistically significant (P lt; 0.05). [ Conclusions ] 1.IL-17A involved in the formation of pulmonary fibrosis , pulmonary fibrosis in the development of pathological processes work. 2.IL-17A alveolitis stage may promote the development of inflammation , and may be directly involved in the late pulmonary fibrosis. 3 Dexamethasone may influence the expression of IL-17A to achieve its therapeutic effect on pulmonary fibrosis .

Related Dissertations

  1. Effect of Curcumin on Hippocampal No-Nos System of Growing Pigs Subjected to Transport Stress,S858.28
  2. The Effect of Glucocorticoid on the Aiolos Expression and the Level of IgE in Asthma Rats,R562.25
  3. The Expression and Clinical Significance of TH17 Cells Related Cytokines in the Patients with Rheumatoid Arthritis,R593.22
  4. Osthol on bleomycin- induced pulmonary fibrosis in rats and its mechanism of,R563.9
  5. Study on Expression of Th17 Cells and Treg Cells in Non-small Cell Lung Cancer in Elderly and the Relationship with Immunosenescence,R734.2
  6. The Dynamic Changes of Serum Cytokines in Paraquat Poisoning Rats and the Artesunate Intervention Studies,R595.4
  7. Effect of Xuebijing on Lung Fibrosis in a Rat Model of Oleic Acid-induced Lung Injury,R563.8
  8. Dexamethasone on skeletal muscle and motor end plates after sciatic nerve injury repair,R651.3
  9. The Th17/treg Regulation of Triptolide on Trinitrobenzene Sulfonic Acid-induced Colitis in Mouse,R285.5
  10. Roles of VEGF and Dexamethasone in the Expressions of SP-B and Tgf-β1 in Type 2 Alveolar Epithelial Cells,R363
  11. Department of Respiratory Medicine, Union Hospital of Tongji Medicine College, Huazhong University of Science and Technology,R563.9
  12. The Effects of Rosiglitazone and Prednisone on the Expression of FIZZ1 in the Pulmonary Fibrosis Induced by Blemycin,R965
  13. The Role of miR-155 in MG and the Effect of Dexamethasone on miR-155,R746.1
  14. The Effect of Dexamethasone on Glucose Metablism of Pregnant Women for Fetal Lung Maturity,R714.2
  15. Change of UGBP in Lung Fibroblasts and Human Bronchial Epithelial and Primary Mechanism,R563
  16. The Protective Effects of Xuebijing Injection on Hyperoxia-induced Lung Injury in Neonatal Rats,R285.5
  17. Nrf2, γ-GCS expression matrine bleomycin- induced pulmonary fibrosis,R285.5
  18. Clinical Study of the Treatment of PF with Matrine Injection,R285
  19. Beidan Yifei on Angiogenesis in Rats with Idiopathic Pulmonary Fibrosis Effects of ET-1 and VEGF,R285
  20. Experimental Investigation on the Neuroprotective Effect of Dexamethasone and Progesterone Therapy for Diffuse Brain Injury in Rats,R965
  21. Regenerative repair experimental study of the effects of anti-inflammatory drugs mandibular defects,R782.2

CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Other
© 2012 www.DissertationTopic.Net  Mobile