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Objective: To observe Liu Wei Di Huang Wan -containing serum TNF-α injured rabbit intervertebral disc the type Ⅰ type II collagen , explore Liuwei dihuang Pill whether the disc extracellular matrix has a protective effect . Methods : 15 rabbits were randomly divided into blank one day group (A ) , 14 days of the blank group (B ) , TNF -α group (C ) , Liu Wei Di Huang Wan serum group (D ) , TNF -α with Liu Wei Di Huang Wan serum group (E ) , surgical T12-L5 disc of T12-L2 nucleus pulposus and annulus line optical microscope , the nucleus pulposus and annulus line L2 -L5 type Ⅰ collagen type II RT-PCR detection and Western blot analysis . Blank group one day the packet after line nucleus pulposus and annulus light microscopy observation , collagen measurement . Establishment of intervertebral disc in vitro culture system , including TNF-α group culture medium , the other containing 5ng/ml of TNF - α ; containing Liu Wei Di Huang Wan serum group, the other containing Liu Wei Di Huang Wan serum 10% ; TNF - α with Liu Wei Di Huang Wan serum group containing for Cd is 5ng / mL TNF-α and Liu Wei Di Huang Wan serum 10% . Collected in the first 14 days of the experiment the remaining four groups of intervertebral disc , respectively for light microscopic observation and collagen measurement . Results: 1 , light microscopy : A, B , E , and the number of C cells and collagen content in turn reduce the RT-PCR and Western blot analysis : group B compared with group A , increased collagen type I , collagen type II decreased ( p lt; 0.01 ) ; C group and the comparison group B , type I collagen increased , the trend is more pronounced decrease in collagen type II ( p lt; 0.01 ) ; E group and C group increased collagen type I , collagen type II decreased trend slowed (p lt; 0.01) Conclusion: Liu Wei Di Huang Wan through against TNF-α inhibition of collagen type II , delay disc degeneration , disc extracellular matrix has a protective effect , experimental kidney treatment of discogenic low back pain basis.
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