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Objective: To observe the micro fat Feikangyin on experimental nonalcoholic fatty liver disease (NAFLD) rat model and to investigate the efficacy of insulin resistance (IR) mechanism. Methods: SD rats 68, male and female, adaptive feeding a week later, were randomly divided into two groups: control group 12, building modules 56 are male and female, blank group to normal food, building modules by Non-alcoholic fatty liver in rats modeling method, I modified fat diet, feeding six weeks later, two randomly selected control group rats and six model groups (male and female) with intraperitoneal anesthesia, the liver do biopsy prompted successful model. Module 50 will be made according male and female rats were randomly divided into five groups: model group, ultra-high-dose group Feikangyin fat, ultra-fat Feikangyin middle dose group, ultra low-dose group Feikangyin fat, metformin groups were 10, followed by gavage treatment, in addition to the general feeding the blank group, the other group will continue to be high-fat diet. After 6 weeks of treatment, were killed overnight fasting for 12 hours, blood and liver tissue, liver and spleen weighed wet weight, calculate the ratio of spleen and liver index; detecting blood ALT, AST, TC, TG, FBG, FFA, FINS, TNF-α, LP, APN; liver homogenate TC, TG, FFA; liver histopathological changes observed; FIRI. Results: 1, in the course of the experiment rats, slow weight gain compared with the normal group, and the fur is not smooth disheveled; blank rats grew rapidly, and the fur smooth; ultra fat Feikangyin each group and metformin group between the model group rats rats with blank between. 2, the model group showed moderate to severe steatosis; ultra fat Feikangyin each group and metformin group liver pathology Histology showed mild to severe steatosis, compared with the model group, the difference was statistically significant (P lt; 0.05); AMD Grease Feikangyin each group compared with the metformin group had no significant difference (P lt; 0.05). 3, ultra-fat ratio Feikangyin groups spleen, liver index, serum ALT, AST, TC, TG, FFA, LP, FIRI, liver homogenates TC, TG, FFA and other aspects than the model group (P lt; 0.05 ), with roughly equal metformin group (P gt; 0.05). 4, each group TNF-α, APN level was not statistically significant (P gt; 0.05). 5, LP FIRI positively correlated with the level (P lt; 0.05), and TNF-α, APN's FIRI no significant correlation (P gt; 0.05). Conclusion 1, IR is closely related with the development of NAFLD, serum levels and FIRI LP was positively correlated with IR interaction, prompting the generation of NAFLD. 2, the experimental results show that the ultrafine fat rat Feikangyin of NAFLD have a good effect, no significant difference with metformin, its efficacy mechanism is mainly manifested in (a) protect the liver cells to restore liver function (2) regulation of lipid metabolism disorders, blood fat (3) reduce liver fat deposition (4) improving IR and high leptin.
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