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Gan Qinghu ear grass (Saxifraga tangutica Engl.) Saxifragaceae saxifrage genus Tibetan name pine drops, bitter and cold, heat treating liver, gallbladder, fever, and trauma. Literature, and no coverage. For the development of the resources of our traditional folk medicine, the papers on the extraction process of the chemical composition and flavonoids Tibetan Gan Qinghu ear grass (Saxifraga tangutica Engl.) And pharmacological studies. By solvent extraction, silica gel column chromatography, polyamide column chromatography, and isolated from the ethanol extract of Tibetan medicine of Gan Qinghu ear grass 12 compounds identified eight compounds structure, chemical and spectroscopic methods: Quercetin (1), quercetin-3-O-beta-D-galactosidase (2), quercetin-3-O-of β-the D-glucoside (3), quercetin-3 - O-β-D-semi lactose-7-O-of β-the D-grape glycoside (4), the β-sitosterol (5), daucosterol (6), n-nonacosane (7), n 30 alkoxy (8). These compounds were isolated from this plant for the first time. Literature, flavonoids saxifrage is a major active components and rich content, therefore, a single factor and orthogonal test ultrasonic assisted extraction Gan Qinghu the Ear Flavnoids the process, the best: 50o C ultrasound under the power 100W conditions with 80% ethanol, solid to liquid ratio of 1:25 (g: mL), extraction of 35min, extracted four times under the same conditions. Obtained Ganqing Hu ear grass extraction rate up 81.73mg / g. Use the the Gan Qinghu ear Flavnoids in antibacterial and anti-aging experiments. The antibacterial experiments extract agar diffusion method and meat broth times than dilution method mass fraction inhibition of six kinds of bacteria. Results showed total flavonoids tested bacteria was inhibited dose effect; fungal part: the weaker the inhibitory effect of low concentrations of yeast, no inhibition of mold. Anti-aging experiments with D-galactose induced aging mice as a model, determination of the liver, brain, heart, kidney, serum tissue superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px ) activity and malondialdehyde (MDA) content. The results show that, the Gan Qinghu ear Flavnoids in enables aging mouse liver, brain, heart, kidney, serum and tissue activity of SOD, GSH-Px activity was significantly higher MDA decreased significantly. Visible, the Gan Qinghu ear Flavnoids can effectively improve the activity of antioxidant enzymes in aging mice organizations, is a more effective anti-aging protection agent.
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