Dissertation > Excellent graduate degree dissertation topics show

The Joint Exposure of Dioctyl Phthalate and Chloroform on Nervous Toxicity in Mouse

Author: QiXiaoLei
Tutor: LiJingShun
School: Jilin University
Course: Occupational and Environmental Health
Keywords: Dioctyl phthalate Chloroform The combined effects of Neurotoxicity
CLC: R114
Type: Master's thesis
Year: 2011
Downloads: 94
Quote: 0
Read: Download Dissertation

Abstract

Dioctyl phthalate (DOP) plasticizer is one of the most important, and usually in the production of soft plastic products, plastic packaging materials, synthetic resin and into the rubber process with a primary plasticizer, with production and use of plastic products, continue to enter the environment, is one of the most widespread environmental pollutants, easy dissolution enriched in vivo, hormone-like effect with the environment, affect human health and the environment, so its human affect more and more people attention. Chlorination is the most commonly used drinking water disinfection, chloroform chlorination byproducts, harmful, many scholars have its toxicity was investigated experimentally. But the DOP and chloroform joint cytotoxicity study is very small. Objective: This article over by the detection of mouse brain lipid oxidation, cerebral organ coefficient, brain neurotransmitters and other indicators to observe the DOP and chloroform combined exposure of neurotoxicity in mice, and explore both the mouse brain The mechanism of injury. Methods: In this study, in accordance with the experimental analysis and design methods of the combined effects of a 2 × 2 factorial analysis grouped. A total of two influencing factors in the experimental design, respectively phthalate, dioctyl phthalate (DOP) and chloroform, both exposed and unexposed two horizontal grouping of four combinations, namely: non-exposed control group, exposure DOP exposure group, only exposed of chloroform exposure group and at the same time exposed the DOP and chloroform exposure group. Each group by gavage in corn oil as a solvent, the DOP group doses of 540mg/Kg, the chloroform group of doses of 112mg/Kg combined exposure group the dose of DOP270mg/Kg chloroform 56mg/kg once a day for 15 days exposure, dislocation, mice were sacrificed and brain organ coefficient was measured brain neurotransmitters (NE, DA and 5-HT), brain tissue oxidative damage (MDA, SOD, GSH-Px), brain cell DNA damage (single cell gel electrophoresis), brain cell cycle and the apoptosis rate. SPSS 16.0 for the results of each set of data for statistical analysis. Numeric variable results (?) ± s, were statistically analyzed using analysis of variance, categorical variables by x2 test. The combined effects of characteristics evaluated using 2 × 2 factorial analysis methods for analysis. The test of significance level alpha = 0.05. Results: 1 gavage for 15 days, the chloroform group of male mice weighed less than the control group (P lt; 0.05), the combined exposure of both female and male body weight of mice, there was no interaction ( p gt; 0.05) DOP and chloroform combined exposure group of male and female mouse brain organ coefficient was no interaction (P gt; 0.05) DOP group, the protein content of the chloroform group and combined exposure group of male and female mouse brain with the control group there was no significant difference (P gt; 0.05) 2, DOP and chloroform combined exposure of mouse brain lipid peroxidation SOD activity in male and female mice the chloroform group were higher than that in control group (P lt; 0.05), the female mice DOP group was higher (P lt ; 0.05), combined exposure group interaction (P lt; 0.05). MDA content in male and female mice DOP group than the control group (P lt; 0.05), the male mice chloroform group was higher (P lt; 0.05), combined exposure there was an interaction (P lt; 0.05 ). The DOP group of GSH-Px activity in male and female mice and chloroform group were higher (P lt; 0.05), male and female mice combined exposure group interaction (P lt; 0.05). 3, DOP and chloroform combined exposure of male and female mice brain tissue of mice brain neurotransmitters 5-HT content DOP group were lower than the control group (P lt; 0.05), the chloroform group were higher than that in the control group ( P lt; 0.05), combined exposure group interaction (P lt; 0.05). NE content in female mice the DOP group and the chloroform group were higher (P lt; 0.05), the combined exposure group and control group significant difference (P lt; 0.05), male mice NE content DOP group was lower than that the control group (P lt; 0.05), chloroform group was higher (P lt; 0.05). DA in female mice DOP group and chloroform group were higher (P lt; 0.05), the male mice DA content chloroform group was higher (P lt; 0.05), combined exposure group interaction (P lt; 0.05). 4, DOP and chloroform combined exposure of mouse brain cell cycle and apoptosis rate of female mice rate of neuronal apoptosis chloroform group and the control group were significantly different (P lt; 0.05), DOP and chloroform combined exposure significant interaction (P lt; 0.05). Male mice rate of neuronal apoptosis chloroform and control groups were significantly different (P lt; 0.05), the S phase of the combined exposure group interaction (P lt; 0.05), a significant interaction between the two combined exposure (P lt; 0.05). 5, DOP and chloroform combined exposure DOP exposure group and chloroform exposure group 0 DNA damage, DNA damage in mouse brain cells in mouse brain cell DNA damage rate is significantly lower than the control group (P lt; 0.05 ); class Ⅰ and class II DNA damage DOP exposure group and chloroform exposure group brain cell DNA damage rate than the control group, the difference was significant (P lt; 0.05), both combined exposure there was an interaction (P lt; 0.05). Conclusion: The results of this study show that the contact DOP and chloroform can be cited the male mice SOD and GSH-Px activity increased MDA content increased; the female mice SOD and GSH-Px activity increased; female and male mice brain nerve the neurotransmitters in 5-HT, NE, DA content increased; increase in male and female mouse brain cell apoptosis rate and an increase in DNA damage, there was an interaction between DOP and chloroform.

Related Dissertations

CLC: > Medicine, health > Preventive Medicine,Health > Health - basic science > Health Toxicology
© 2012 www.DissertationTopic.Net Mobile