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Günther (Cynoglossus semilaevis) is an economically important fish, with fast growth and so on. Myostatin (MSTN), muscle endostatin, also known as transforming growth factor 8 (GDF-8), transforming growth factor belonging to β (TGF-β) family members. In mammals and other species in the main in the developing and adult individuals skeletal muscle plays a role in inhibiting muscle growth. Follistatin (FST) of follistatin, a cysteine-rich glycosylated single chain polypeptide, which comprises MSTN included for the TGF-β family members is an important regulatory protein, play of Myostatin inhibition; Activin II type B receptor (ActRIIB) is a transmembrane serine / threonine kinase receptor family one, MSTN first combine to form complexes with ActRIIB activation of downstream Smad transduction proteins, start signaling pathways. In this study, by homologous cloning and RACE (Rapid amplification of cDNA ends) technology to obtain FST and ActRIIB full length cDNA, the use of biological software for gene prediction bioinformatics analysis and real-time fluorescence quantitative techniques by means of two genes different tissues and developmental stages of the temporal and spatial expression were studied. 1, Günther's Follistatin gene cloning and expression analysis in GenBank submitted Fish Follistatin sequence conservation area design degenerate primers to obtain core fragment by RACE technique to obtain the 3 'and 5' ends by stitching obtained Günther FST cDNA full length 1373bp, ORF was 966bp, also includes 16 bp of 5'UTR, 417bp of 3'UTR. This gene encodes a 321 amino acid protein containing precursors, the predicted molecular weight of 35.5kD, isoelectric point of 8.12, the protein contains an N-terminal 31 amino acid residues of the signal peptide, is a secreted type protein. Precursor protein comprising the signal peptide region, N-terminal domain, Follistatin domain Ⅰ, Follistatin domain Ⅱ, Follistatin Ⅲ domain and a C-terminal domain. Comparative analysis shows that FST C.semilaevi amino acid sequence homology with other species FST higher, and its domain Ⅰ and Ⅱ more conservative, and rich in conserved cysteine ??residues. FST in Günther 10 kinds of tissues were expressed, including in the testis, ovary, spleen express higher levels; developmental stages FST expression showed some volatility, with the expression of MSTN similar trend and show some interaction , suggesting that the FST in the early development played a role. 2, Günther's ActRIIB gene cloning and expression analysis of ActRIIB is Myostatin pathway important receptor protein by homology cloning and RACE get Günther's ActRIIB cDNA is 2053bp, which ORF sequence is 1539bp, also includes 195 bp of 5'UTR, 325 bp of the 3'UTR. The gene encodes 512 amino acids, proteins predicted molecular weight of about 58.12 KD, isoelectric point of 5.80, N terminus a 24 amino acid residues of the signal peptide, transmembrane proteins. The protein contains distinct serine / threonine kinase active site signature sequence, and the extracellular domain, transmembrane domain, a serine / threonine kinase domain in three important domains. In Günther all detected 10 kinds of organizations have different levels observed AcvRIIB expression, but there is the expression of the difference; ActRIIB is maternally expressed, and MSTN compared to the developmental stages of expression is low in weight and more stable.
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