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Exogenous Trehalose Improve Arabidopsis Thaliana Salt Tolerance

Author: WangHongLing
Tutor: TangZhongHua
School: Northeast Forestry University
Course: Botany
Keywords: Salt stress Trehalose Arabidopsis ROS Ion balance
CLC: Q945.78
Type: Master's thesis
Year: 2011
Downloads: 61
Quote: 0
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Abstract


Inorganic stress can directly or indirectly affect plant growth and yield, salt stress for plants is one of the most common inorganic stress. Salt resistance of plants A series of adaptive physiological processes caused by the complexity of the phenomenon of life. Experience to make the appropriate response to a variety of stress signals, such as taken from the growth and development of the external morphology, physiological and biochemical aspects of defense strategies to improve the resistance to withstand drought stress, salinity stress and chilling injury and other environmental stresses, plants. High salt stress is a serious plant injury inorganic stress conditions, plant in continuous evolution in its own body has formed a relatively complete set of defense systems, including the regulation of homeostasis, generate osmotic adjustment substance poisoned ion regionalization and reactive oxygen species (ROS) scavenging system. Trehalose is a widespread in nature the presence of a non-reducing disaccharide. Widespread in bacteria, fungi and insects as a source of carbon and still kind of stress protection material has a protective effect on a variety of external stress, but difficult to measure trehalose in higher plants, only a trace substances . Some research at home and abroad have proved trehalose has good resistance to improve drought resistance, for example, can be effective, but less salt resistance of trehalose and trehalose salt tolerance mechanism is not very clear. In our study found that the amount of exogenous trehalose can effectively improve the wild-type Arabidopsis (Col-0) both seedlings and seedling salt resistance. Arabidopsis thaliana seedlings growing under salt stress from external morphology study found that exogenous trehalose mainly by increasing the flowering rate and spend quite a height, increasing the water content of the fresh weight of the aerial parts and rosette leaves better. Plants peroxide (ROS) accumulation of excess is an important signal of the plant has been coerced by the determination of ROS in the apical and rosette leaves come in this experiment, trehalose can effectively clear plants salt stress and excessive ROS generated mainly by regulating the body's antioxidant enzyme activity, such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and ascorbic acid over peroxidase (APX). In addition, we also found that an important role of trehalose is able to maintain the plants of K / Na dynamic equilibrium. High salt stress, exogenous trehalose able to reduce the Na content in Arabidopsis leaves and flowers very also able to reduce the loss of K, to improve the salt resistance of plants to achieve the purpose of maintain K / Na homeostasis. Salt stress in varying degrees for the the plants penetrate regulation also different substances, soluble sugar is the main osmoregulation substances, low-salt stress can reduce the Arabidopsis leaf soluble sugars (such as glucose and sucrose) content, and high content under salt stress. Exogenous trehalose to improve due to salt stress for soluble sugar content of plants to improve the osmotic potential of plants to improve plant salt resistance. But it the trehalose This role is not very clear, and still need to do further research it. In summary, the appropriate concentration of trehalose can effectively improve the salt resistance of the wild-type Arabidopsis thaliana, and found that in the test of 1mM trehalose acts as a significant. In addition, we use the same non-reducing disaccharide sucrose as a control experiment, the results show that the corresponding concentration of sucrose is not effectively improve the salt resistance of plants.

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