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Screening and Identification of Microorganisms Using the Phosphite in the Sediment from Lake Taihu

Author: CaoRuiXiang
Tutor: GengJinJu
School: Nanjing University
Course: Environmental Engineering
Keywords: Sediment in Taihu Lake Phosphite Microorganism Filter Appraisal Phosphorus metabolism Mechanism
CLC: X524
Type: Master's thesis
Year: 2011
Downloads: 33
Quote: 0
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Abstract


Has long been that cheap phosphorus (less than pentavalent phosphorus) is not involved in the biogeochemical cycling of phosphorus, a recent study proved cheap phosphorus forms, such as: phosphite, hypophosphite, phosphine, is also an important source of phosphorus in the environment, Biochemistry earth cycle involved in the phosphorus environment, some microorganisms in the case of aerobic or anaerobic using cheap phosphate as a phosphorus source growth, or from the low phosphate oxidation process energy. Phosphorus is important biogenic elements, lake eutrophication limiting nutrient, little studied lakes in the presence of cheap phosphorus bioavailable on cheap phosphorus in eutrophic lake microbial metabolic processes , is of great significance for revealing the the lake phosphorus biogeochemical cycling mechanism, to explore the bioavailability of the different phosphorus forms. In Taihu Lake, screening is the only low-cost phosphate phosphorus source of microbial growth, morphology further screened microbial detection, 16S rDNA identification and optimization of culture conditions, and their metabolism is low The price of phosphate mechanism is discussed. The main findings are as follows: 1 from sediment in Taihu Lake failed to screen out inferior race phosphate (H2PO2-, 1 price) as the sole source of phosphorus microbial screened 10 phosphite (H2PO3-3 price) a microorganism of the sole phosphorus source, further wherein the three bacterial (P1 bacteria, P2 bacteria and P3 bacteria), the generation of P1 bacteria, P2 and P3 strain shorter G = 1.5h, P1 bacteria, P2 and P3 maximum bacterial density of the bacteria were 1095 mg / L, 1231 mg / L and 1189 mg / L 2.P1 bacteria, P2 of bacteria and P3 bacteria are gram-negative aerobic bacilli, scanning electron microscopy to P1 of bacteria and P2 bacteria bacterial surface smooth, viscous P3 bacteria bacterial surface. Length of the P1 the bacteria, P2 of bacteria and P3 bacteria, 16S rDNA PCR amplification products of approximately 1.5 kb was measured strains of 16S rDNA sequences in Genbank have sequence homology comparison, the results show that strain P2 Pseudomonas (Pseudomonas sp.), but with the known Pseudomonas homology is not high, P1 strain and P3 strain with all known strains homology are low, new bacteria belonging unknown. The 3.P1 bacteria and P2 bacteria optimal culture conditions: optimum growth temperature is 30 ℃, the optimum inoculum size of 40mg / L. P1 bacteria optimum pH range of 6.5-6.8, P2 strains optimum pH range of 6.8 ~ 7.0. 4.P1 and P2 strain can be oxidation of the phosphite to orthophosphates highest phosphite oxidation rate of 1.7%. Found that through the detection of the medium of alkaline phosphatase (BAP) activity in culture Ji Neiya phosphate concentration is lower, BAP higher activity, wherein P1 bacteria more apparent. P1 and P2 bacteria is a high concentration of phosphite growth promoting type bacteria. P1 and P2 strains are phosphites for the growth of bacteria in the sole phosphorus source, in orthophosphates, hypophosphites, phytic acid and glyphosate phosphorus source not growth, presumably phosphites of both P1 bacteria and P2 bacteria growth and a source of phosphorus, and also the P1 of bacteria and P2 bacteria need to obtain energy from the oxidation of phosphite process.

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CLC: > Environmental science, safety science > Environmental pollution and its prevention > Water pollution and its control > Lakes and reservoirs
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