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A Study on Vegetative Propagation of Nitraria Sibirica Pall. Chosen Clones and Electrical Impedance of Ulmus Pumila Seedlings

Author: ShiRuiTing
Tutor: LiYue
School: Beijing Forestry University
Course: Tree Genetics and Breeding
Keywords: Nitraria Clone Clone U. pumila Impedance spectroscopy
CLC: S793
Type: Master's thesis
Year: 2011
Downloads: 36
Quote: 1
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Abstract


The test system to study the Nitraria clones tissue culture and softwood cuttings , including the sterilization of explants , aseptic subculture , rooting and hormone softwood cuttings . Study salinity stress on the electrical impedance of the impact on the the U. pumila family growth , study the of U. pumila salt tolerance of its relevant indicators provide a new basis . The results are as follows : 1 , the best method of disinfection of explants explants collected directly into clean benches for disinfection , use 70 % alcohol soaked 25s , disinfected with 0.1% HgCl2 8min lowest contamination rate . . 2 of research the 6-BA.KT.NAA.IBA Plantlet 6-BA and IBA four clonal proliferation of cultured suitable plant growth regulator . 3, N6 medium than MS , B5 , WPM more suitable subculture proliferation of cultured . Shuanggang 01 and Shuanggang 02 following the cultured mitogen and auxin ratio are BA 1.5mg / L IBA 0.5 mg / L; Shuanggang 03 subculture mitogen and auxin ratio of BA 1.0mg / L IBA 0.5 mg / L; the Shuanggang 04 best following the cultured mitogen and auxin ratio for BA 1.0mg / L IBA 0.2 mg/L.5 four asexual Department of rooting medium are 1/ 2 MS NAA 0.5 mg/L.6 Nitraria clones softwood cuttings rooting rate and rooting number of very significant impact, no impact on the main root length ; types of plant regulator concentration and treatment time had a significant effect on rooting rate , but no effect on root number and taproot length . Shuanggang 04 best rooting , rooting combination of 250mg / L of ABT treatment 2h . , With a salinity concentration increased stress , the extracellular resistance relaxation time of each family goodbye to the characteristics of the small basic show decreases and then increases ; pedigree pedigree and pedigree intracellular resistance presented the M \8, Re and Ri , τ , Ri and τ rendered extremely extremely significant with a positive correlation ; ( ? ) Ri, τ are rendered extremely significant with negative correlation ; (?) With Pn also presented negative was significantly correlated ; Pn and τ presents a significant positive correlation . Extracellular resistance and relaxation time can effectively said the degree of of U. pumila salinity stress .

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved shrubs
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