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Analysis of Drought Tolerance and Water Saving Ability in DREB1A Transgenic Plants of Ground Cover Chrysanthemum ’Fall Color’

Author: YuYang
Tutor: HongBo
School: Northeast Forestry University
Course: Ornamental Plants and Horticulture
Keywords: AtDREB1A gene By daisy Drought Resistance
CLC: S682.11
Type: Master's thesis
Year: 2011
Downloads: 56
Quote: 0
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Abstract


Chrysanthemum (Ground cover Chrysanthemum) Chrysanthemum (Chrysanthemum morifolium) in a variety of groups with low plants, vigorous growth, strong branches, crown neat, brightly colored, urban landscaping widely used. Multiple cities are increasingly high temperatures, drought and other environmental factors severely restricted the chrysanthemum cultivation applications, especially in the promotion of of our northern Gaozao dryland been limited. In this study, has been obtained by chrysanthemum turn drought-resistant transgenic plants, drought-resistant water-saving research through its order for the chrysanthemum drought tolerance screening of new varieties work laid the theoretical foundation, while filtering out the good viewing, drought strong ground chrysanthemum new germplasm. This study have been obtained with multiple stress resistance of revolution DREB1A gene ground chrysanthemum six strains as test material, to not genetically modified wild-type plants 'WT' control. By way of tissue culture propagation, and the potted seedlings of each strain were carried out under ambient conditions and under high temperature conditions of the water stress treatment. By measuring the variation of the indicators of physiological indicators and water use efficiency under different water stress time exploring drought-resistant water-saving features of the various strains in the soil drought, as well as air and environmental conditions such as drought. Finally, analysis of variance and a comprehensive evaluation of the different strains of membership function value statistics. Status of a genetically modified strain of seed germination in different concentrations of PEG solution were measured under high temperature stress membrane permeability, water loss rate of detached leaves, leaf relative water saturation deficit, wilting index, biomass and plant height stress index 7 associated with drought resistance physiological and morphological indicators; measured the various strains Chrysanthemum soil limit water content, soil limits water consumption, water use efficiency, leaf relative water content and water use efficiency indicators. The study results show that: under water stress conditions, compared to the the various strains turn DREB1A gene with the wild-type plants significant difference except '18-5 'strain, and the remaining five transgenic lines comprehensive drought-resistant water-saving capacity are better than the wild-type 'WT' strain. Which, '28-46 ', '28-45', '28-10 '3 lines 'WT' significant differences, and no significant difference between these five lines. Weak drought-resistant water-saving '18-5 'lines with wild-type' WT 'strain differences not significant. It can be confirmed that exogenous AtDREB1A gene in chrysanthemum on heterologous expression to improve the stress tolerance of transgenic plants to high temperature, drought and other adverse environmental.

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Perennial Flowers > Perennial Flowers Class > Chrysanthemum
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