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In this study, the determination of Streptomyces 769 antibacterial spectrum and growth curves based on transformed into E. coli-Streptomyces genus indirectly interested in an aggregate transfer are two ways to establish a genetic transformation system of Streptomyces 769 and optimize the conditions for genetic transformation. To obtain the following results: 1. Vitro antibacterial test showed that the The Gongzhuling neomycin the bacteria Streptomyces (Streptomyces) 769 plant pathogenic fungi has a broad-spectrum inhibitory activity of the rice blast fungus (Magnaporthe grisea), corn turcica ( The Exserohilum turcicum), sorghum Ustilago (Sphacelotheca cruenta), corn ear rot (Gibberella zeae), green onions the purpura bacteria (Alternaria Porri), tomato anthracnose fungus (Colletotrichum coccodes) and other 16 kinds of plant pathogenic fungi were significantly inhibitory effect, The different types of pathogen sensitivity there are some differences. 2 In this study, the optimum liquid medium of Streptomyces 769 to filter, by measuring the absorbance values, when the mycelium growth, the broth biological activity of the three indicators identified by its cultured with shaking in YEME medium shaker growth curve :0-36h delay of growth ,36-84h ,84-108h logarithmic growth phase for the stability of 108h decline phase. Study conjugal transfer, electroporation genetic transformation methods of influencing factors and conversion conditions, to establish a stable genetic transformation of Streptomyces 769 system. The gene integrated plasmid pSET152 of plasmid, ET12567 (pUZ8002, pSET152) donor, 769 spores of Streptomyces receptor, on MS medium conjugal transfer, the proportion of the donor strain and the recipient strain 10:1, spores pre-germination conditions for the 50 ° C heat shock 15min, covering 16-18h, the conversion efficiency of about 0.3 × 10-5. Gene acc (3) of the resistance to apramycin IV as the target gene by PCR validation successfully transferred into the plasmid pSET152 Streptomyces 769. At the same time, the conditions of preparation of the test reference Micromonospora transformed Streptomyces 769 competent cells, the type of electric field intensity of the electric transformation experiments. The results showed that; 2.5kV electrical pulse intensity, electric shock time of 3.1 milliseconds, antibiotic concentration 15ug/ml, the 769 Streptomyces concentration of 106/ml, Gao 1 medium transformants by PCR validation plasmid transferred pSET152 to Streptomyces 769, conversion efficiency and stability needs to be improved, the power conversion conditions to be further investigated.
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