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Study on the Sensitivity to Mesotrione in Setaria Glauca(L.)Beauv. and Setaria Faberii Herrm.

Author: LiuShiYang
Tutor: LiXiangJu
School: Chinese Academy of Agricultural Sciences
Course: Weed Science
Keywords: Gold Setaria Giant foxtail Nitrate Sulcotrione Chloroplast pigment Glutathione-S- transferase HPPD gene
CLC: S451
Type: Master's thesis
Year: 2011
Downloads: 58
Quote: 0
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Abstract


Poaceae (Gramineae) the gold green foxtail (Setaria glauca (L.) Beauv.) And giant foxtail (Setaria faberii Herrm) are Setaria (Setaria Beauv) annual weeds, widely distributed all over the country. Major hazards corn and other the Summer crop fields and orchards. The Setaria weeds in our corn growing areas higher frequency of serious harm to corn production. The nitrate Sulcotrione newly developed herbicides kill weeds by inhibiting Weed HPPD activity, with high efficiency, broad spectrum herbicide and corn safety features. However, in the use of the process in the field, it is found that the presence of nitrate tolerance of sulcotrione between Setaria different species difference. In this study, two kinds - Setaria Setaria Setaria as the research object, the first bioassay method to study spraying nitrate Sulcotrione two kinds of differences in phenotype; thus using molecular biology Technical fellowship green foxtail, giant foxtail HPPD gene sequence differences; same time, physiological and biochemical analysis of gold green foxtail and giant foxtail two kinds of chloroplast pigment and GSTs nitrate Sulcotrione processing. The main results are as follows: 1 indoor potted plants law test, studied two Setaria Setaria Setaria weeds on the the nitrate Sulcotrione the difference in sensitivity. The study found that the same dose of nitrate Sulcotrione the gold green foxtail of nitrate Sulcotrione the sensitivity to be higher than the giant foxtail the GR50 as of 180.8 g ai · ha -1 is the the giant foxtail GR 50 values ??nearly 5 times. 2 has been obtained two bristlegrass HPPD gene length 589bp, encoding 141 amino acid residues, green foxtail and giant foxtail 80,81 and 131 amino acid type, namely alanine (Ala) and valine (Val ); valine and alanine; leucine (Leu) and serine (Ser). Chloroplast pigment content test showed that the nitrate Sulcotrione processing Setaria Setaria chloroplast pigment content with time showed a downward trend, but the gold Setaria these indicators decline significantly larger than the decrease of the giant foxtail . After 14 days nitrate Sulcotrione process, of giant foxtail chloroplast pigment content level gradually returned to the level before the chemical treatments, but the gold Setaria dealt with accordingly after application, the longer the lower contents of pigments. 4 of GSTs in vivo activity test indicated that no drug treatment, the GST activity of the giant foxtail 0.8 0 .85, while gold Setaria GST activity 0.6 0 .65. After nitrate Sulcotrione processing, giant foxtail and gold Setaria GST activity were significantly increased. But significant reduction in elevated gold Setaria Setaria. After treatment,, gold green foxtail GSTs activity was significantly decreased relative activity values ??decrease. To 21 d, gold Setaria GSTs activity value to 0.50. 9 days when giant foxtail GSTs activity still increased. The first 14 days, the value of GST activity was significantly reduced. Medication after 21 days, giant foxtail GSTs activity to restore the original level. The different points are gold green foxtail and giant foxtail grass nitrate sulfur-tolerant differences. The results of this study can not only enrich Setaria on the nitrate Sulcotrione the tolerance mechanism of the theory, and weed management also has practical significance.

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CLC: > Agricultural Sciences > Plant Protection > Harmful plants and their removal > Weeds
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