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Alien plants risk early warning is important work biosafety rich intrusion risk assessment is to achieve early warning indicators and key. Involving DNA C-values ??and genome size of nuclear DNA content of plant invasion risk assessment may be an important indicator. Since the nuclear DNA content in different groups of plants height variability, grasp as much of the plant's nuclear DNA content for the scientific use of this indicator has important practical value. Therefore, this paper flow cytometry East region is an important invasive species of plants and their relatives were nuclear DNA content, explores standards, the nuclei isolation buffer, etc. The results of the nuclear DNA content of effects and morphological diversity nuclear DNA content of plant species under the variation. The main results are as follows: 1. Different standard samples of nuclear DNA content has a significant impact, the choice of standard samples and test samples of nuclear DNA content should be similar, but there are significant differences, which need to filter out through the pre-test sample appropriate standards; 2. despite the reported nuclear isolation buffer variety, this study shows, Otto buffer suitable for the separation of the nucleus herbs; 3, a total of 10 families were determined East 34 kinds of herbs nuclear DNA content , won their ploidy. First reported goldenrod (Solidago decurens), small canadensis (Conyza canadensis), Punta year (Erigeron annuus), Sumatran fleabane (Conyza sumatrensis), fleabane (Conyza japonica), lettuce (Lactuca sativa var. Ramosa), Black sorghum (Sorghum almum), Sorghum (Sorghum sudanese × Sorghum bicoloy), millet (Setaria italica), safflower sorrel (Oxalis corymbosa), wood sorrel (Oxalis corniculata), purple leaf shamrock (Oxalis triangularis var . purpurea), Ageratum (Ageratum conyzoides), Bear Ear grass (Ageratum houstonianum), Pterocypsela (Pterocypsela indica), multifidus Pterocypsela (Pterocypselalaciniata), Plantago (Plantago virginica), Pinellia (Pinellia ternata) , crepidioides (Gynura crepidioides), Jerusalem artichoke (Helianthus tuberosus), fake rice (Leersia japonica), India weld dish (Rorippa indica), chervil (Apium leptophyllum), thorn fruit buttercup (Ranunculus muricatus), speedwell (Veronica polita ) and ivyleaf (Pharbitis hederacea) and other 26 kinds of plants, nuclear DNA content and ploidy, get their DNA 2C values ??and their genome size; Solidago canadensis (Solidago canadensis), poison lettuce (Lactuca serriola) , sorghum (Sorghum bicolor), sorghum (S.halepense), gold dog tail (Setaria glauca), oats (Avena sativa), wild oat (Avena fatua), shepherd's purse (Capsella bursa-pastoris) and other plants of nuclear DNA content were measured, the results with previous similar reports, reflecting the results of this experiment are more reliable; 4, on the North American front, Pinellia and other plants found in nuclear DNA content, there is some morphological differences of the individual, the nuclear DNA content there are also differences, but did not reach statistically significant levels. This shows the presence of nuclear DNA content variation planted, it also shows that nuclear DNA content for a species in terms of relative stability. 5, speedwell (Veronica didyma) (0.643pg/2c, genome size 0.643pg), chervil (0.774pg/2c, genome size 0.387pg), India Rorippa (1.050pg/2c, genome size 0.525pg), fake rice (1.59pg/2c, genome size 0.795pg), crepidioides (3.825pg/2c, genome size 1.915pg), North American car before (1.177pg/2c, genome size 0.588pg), Bear Ear grass (1.393pg/2c , genome size 0.697pg), Ageratum (1.273pg/2c, genome size 0.637pg), ivyleaf (1.348pg/2c, genome size 0.674pg) and other plants have relatively low nuclear DNA content, lower than the world malignant weeds mean nuclear DNA content (2.69pg/2c, genome size 1.65pg), reflect these plants may have strong invasion capacity, which more in line with the actual situation; compare invasive species and invasive species nuclear non-relatives DNA content found poison lettuce (2.13pg/2c, genome size 0.533pg), sorghum (3.345pg/2c, genome size 0.836pg), safflower sorrel (1.72pg/2c, genome size 0.86pg) than near edge of the non-invasive species have significantly lower nuclear DNA content; however also found wild oats (23.938pg/2c, genome size 3.99pg), gold dog tail (5.851pg/2c, genome size 1.17pg), Sumatran fleabane ( 4.807pg/2c, genome size 1.203pg), Solidago canadensis (5.867pg/2c, genome size 2.493pg) nuclear DNA content and its relative non-invasive species are similar, or higher than the latter. 6, Pinellia nuclear DNA 2C value (8.444pg) slightly higher than the world's major weeds (8.18pg), but its genome size (2.029pg) and smaller than the latter (2.46pg); Wild Chrysanthemum is exotic cultivated plants, its DNA 2C value than the world weed high, but lower than the value of the world's major weeds DNA 2C, its genome size than the lower world, weed, therefore, to strengthen the monitoring of these exotic plants. 7, first discovered haploid speedwell, pentaploid purple Oxalis, pentaploid gold dog tail, thorn fruit buttercup hexaploid and tetraploid Banxia fold phenomenon.
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