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Preparation of the purpose of of this experimental to take advantage of the Femur of Pigs new type of acellular bone matrix (acellular bone extracellular matrix, ABECM) material and dehydroxylation of the the cells bone matrix - chitosan (Chitosan, CS) bone tissue engineering composite porous stent, detection the the of its physical and chemical performance and with the bio-compatible The sexual, to explore its become the the the the the feasibility of of the the the stent material of the the of the bone tissue engineering, to to provide experimental basis for the the research of the tissue engineering the bone stent of the. Method (1) adopting Triton X-100 and Titanium alkyl over sodium sulfate (sodium dodecyl sulfate, SDS), etc. Joint off the processing, of the the cell method pairs of the the backbone of the pig stocks conducted and an analysis of the a pairs ABECM material for qualitative perspectives.. Application of solution blending, freeze-drying the legal system 备 de-the composite stent of the cells bone matrix / chitosan (ABECM / CS) bone tissue engineering, carried out the histological on the the composite porous stent, characteristics of immune organizations chemical, scanning electron mirror, porosity of, mechanics performance, etc. were detected and analyzed. (2) separation, cultivate the the the mesenchymal stem cells in in the rabbit bone marrow mesenchymal stem of the (Bone marrow cells BMSCs), of of will be the cells with the stent in in the passaged composite of common cultivate, the cells was observed adhesion, the growth of, with the proliferation the situation; adopt the the leaching liquor of the the observe the material of the MTT method carried out the the activity of of the cell toxicity experimental the cells were observed in the 1, 3, 5, 7 on day; the the the material leaching liquor with the the the diluted blood was observed after in the mixed centrifugal red blood cell hemolysis the situation, detection the OD value of to calculate the relative the hemolysis rate of; will be material leaching liquor by the intravenous injected into The rabbit vivo subjected to heat original experiments, in the the a predetermined within the time, Observation on the rabbit This body temperature change can. Results (1) ABECM materials and ABECM-CS HE staining of the composite scaffold, residual cells was observed in the Hoechest33258 fluorescence dyeing there is no, toluidine Lan, Amidst the burning ethnic red O staining, the alizarin red the dyeing dyeing-positive, Ⅰ of collagen type, of bone morphogenetic occurrence of protein-2 antibody immune organizations chemical dyeing-positive. The scanning electric mirror and the Micro-CT display the scaffold has a porous of the ABECM-CS structure, pore size 50-200μm, porosity of (53.50 ± 4.23)%, mechanics the detection stent in the dry state under the longitudinal compression modulus of for the (33.23 ± 14.45) Mpa, stent The the the under a compression of modulus in in the wet state for the (2.27 ± 1.13) Mpa. The (2) cells - stent composite cultivate to the trials have shown that cell adhesion with the the growth of a good, the life and death of cells were stained with visible the adhesion living cells on the in the stent, the stent the surrounding cell growth an active the cells stretched out connected to the the pseudopodia mutual between the, and there are matrix ingredients produce. The cells toxicity trials have shown that that the, cultivate the 1, 3, 5, 7, days after the of each period of time to three groups of the OD value of twenty-two compare the the gt of (P; 0.05), no statistically significant differences, indicating that the non-toxic in the material. In the the in the the hemolysis test, the 3.0% of the observation of the of the hemolysis rate was of of the experimental material, in the the the the 5% the scope of within the the of the the standard on Biological material value hemolysis rate of, are prompted to the that no hemolysis the phenomenon of occurrence of. The thermal-of the original experimental the results of display that the each rabbit body temperature elevated were lower than the 0.6 ℃, and the 3 rabbit body temperature elevated the the total degree of is lower than by 1.4 ℃, to in line with the pyrogen detection the provisions of, composite materials-free the the role of induced thermo. ABECM Materials and CS solution blending of the obtained by by the Conclusion (1) considered by a Joint acellular processing, composite porous stent of the by the Preparation of of the by the freeze-drying method ABECM / CS to go cells thoroughly, to retain the the main component of of the acellular bone matrix, possess the an appropriate pore size and pore rate of; (2) ABECM / CS composite scaffold non-cells toxicity, the no hemolysis reaction, non-thermal immunogenicity, cells - the stent composite cultivate The trials have shown that the a good cells the growth of with the adhesion, the to has a the good of the biological the safety the sexual and cells of of the compatibility of, can be used as build the the the of the stent carrier material of of the of tissue engineering bone.
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