Dissertation > Excellent graduate degree dissertation topics show

Teh Effect of Different Treated Pure Titanium Surfaces on the Growth of Osteoblasts

Author: MeYuan
Tutor: DongFuSheng；RenGuiYun
School: Hebei Medical University
Course: Clinical Stomatology
Keywords: Pure titanium surfaces blasted-etched osteoblasts culture in vitro biological property
CLC: R783.1
Type: Master's thesis
Year: 2011
Downloads: 70
Quote: 0
Read: Download Dissertation

Abstract

Objective: Pure titanium and titanium alloy have fine biocompatibility. By changing the surface morphology and structure of implants, the surface biological activity of implants can be advanced and the formation of osseointegration of implants can be improved. The growth activity and biological function of osteoblasts, the surface characteristics of implant materials which include surface roughness, surface hydrophilicity and chemical composition of surfaces and the forced environment of implants affect osseointegration of implants. The blasted-etched treatment which can enhance the surface roughness of implants not only strengthen contiguous areas and styles of implants and bone tissues around implants, but also enhance biological functions of osteoblasts. The blasted-etched treatment is better to the formation of osseointegration of implants. Osteoblasts are important cells forming bone tissue surrounding implants. TiO₂ blasted and HCl/H₂SO₄ etched pure titanium surfaces, Al₂O₃ blasted and HCl/H₂SO₄ etched pure titanium surfaces and untreated smooth surfaces were used in this study. To provide a more favorable scientific experimental basis for the development of clinical implants, cultivation in vitro and identification of SD rats osteoblasts, infolding morphology of osteoblasts observed by SEM on three kinds of surfaces as well as proliferating activity of osteoblasts by MTT assay were used.Methods:1 Preparation and grouping of titanium discsTwenty-four slices pure titanium discs were prepared with 10mm in diameter, 1mm thickness and 0.8μm roughness, divided into 3 groups, and 8 slices each.Group A （TiO₂ particle blasted and HCl/H₂SO₄ etching group）: titanium discs were blasted TiO₂ particle with 5Kpa atmospheric pressure and etched by HCl/H₂SO₄ mixed liquor.Group B （Al₂O₃ particle blasted and HCl/H₂SO₄ etching group）: titanium discs were blasted Al₂O₃ particle with 5Kpa atmospheric pressure and etched by HCl/H₂SO₄ mixed liquor.Group C （smooth group）: the surface without any treatment.2 The morphology of three kinds of different treated pure titanium surfaces by SEMTiO₂ blasted-etched titanium discs, Al₂O₃ blasted-etched titanium discs and untreated smooth titanium discs were observed by SEM.3 The detachment, cultivation, passage and identification of osteoblasts Skulls of SD rats for born 24 hours were obtained and cutted into trivial bone flaps. Original passage osteoblasts were gained with“Twice enzyme digestion method”and“quick difference adherence approach”. Osteoblasts were digested and generated by 0.25% pancreatin once they expended on the whole space of the culture flask. The second passage osteoblasts were fabricated into cell slices with hematoxylin and eosin stain as well as Immunocytochemical stain of BMP-II albumen, hereby were used to identificate resource of osteoblasts we got.4 The cultivation of osteoblasts on different treated pure titanium surfaceThe second passage fine growing osteoblasts were produced into unicell suspension （the density of unicell suspension is 8×10⁵ piece/ml） and respectively vaccinated on TiO₂ blasted-etched titanium surfaces, Al₂O₃ blasted-etched titanium surfaces, smooth titanium surfaces and controlled coverslips surfaces. Three groups of titanium discs and coverslips were respectively putted into 24 holes training boards. After co-cultivation of osteoblasts and four kinds of surfaces for 3 days in 24 holes training boards, these surfaces were observed by SEM and cytoactive of osteoblasts was determined by MTT assay.5 The morphology of osteoblasts on TiO₂ blasted-etched titanium discs, Al₂O₃ blasted-etched titanium discs, smooth titanium discs and coverslips surfaces observed by SEMThe second passage fine growing osteoblasts were produced into unicell suspension （the density of unicell suspension is 8×10⁵ piece/ml） and respectively vaccinated on Group A surfaces, Group B surfaces, Group C surfaces and controlled coverslips surfaces. After co-cultivation of osteoblasts and four kinds of surfaces for 3 days in 24 holes training boards, the morphology of osteoblasts on these surfaces was observed by SEM.6 The determination of cytoactive of osteoblasts on three kinds of titanium discs surfaces by MTT assaySamples of TiO₂ blasted-etched titanium, Al₂O₃ blasted-etched titanium and smooth titanium discs were respectively put into 24 holes training boards after hyperthermia and hyperpiemia disinfected, and six parallel samples were set up in each group. The second passage cell suspensions of SD rats osteoblasts was vaccinated on every titanium surfaces of three groups in each hole （the vaccinated density is 7×104 piece/hole）. After co-cultivation of osteoblasts and titanium discs for 3 days, the absorbance value on three kinds of titanium discs surfaces was respectively determined by ELIASA （Enzyme-linked immunosorbent tag analyzer） at 490 nm wavelengths.7 Statistical treatmentsThe absorbance values that was determination by MTT assay was treated with SPSS 13.0 statistical soft. The comparison among three groups was used one-Way ANOVA and P <0.05 indicated results had statistical differences.Results:1 The morphology of three kinds of different treated pure titanium surfaces by SEMThe first and second grade of pouches was formed on surfaces of TiO₂ blasted-etched titanium discs and Al2O3 blasted-etched titanium. The morphology of these pouches on the two surfaces was similar, but superficialis pouches were formed on smooth titanium discs surfaces.2 The identification of osteoblastsThe original passage cells which presented appearances of fibroblasts on invert microscope were irregular fusiform or polygon. The transferred passage cells could show up phenomenon of overlapping growth.Cell nucleus was blue and cytolymph was red with HE stain of osteoblasts. Appearances of osteoblasts were irregular fusiform or polygon. Cell nucleus of osteoblasts presented orbicular-ovate and it could be observed1 to 3 nucleoli in some osteoblasts.Slices of osteoblasts were stained with Immunocytochemical stain of BMP-II albumen. It was observed that in various visual fields most osteoblasts were male cells whose cytolymph were buffy-stain or brown-stain.The cells were osteoblasts with HE stain and Immunocytochemical stain of BMP-II albumen.3 The morphology of osteoblasts on TiO₂ blasted-etched titanium discs, Al₂O₃ blasted-etched titanium discs, smooth titanium discs and coverslips surfaces observed by SEMOsteoblasts formed cell layers of overlapping growth on TiO₂ blasted-etched titanium, Al₂O₃ blasted-etched titanium and smooth titanium discs surfaces. Osteoblasts presented single growth on covers -lips surfaces. The numbers of growing osteoblasts were more on TiO₂ blasted-etched titanium, Al₂O₃ blasted-etched titanium and smooth titanium discs surfaces than on coverslips surfaces.The depths of cell layers on TiO₂ blasted-etched titanium and Al₂O₃ blasted-etched titanium were more than on smooth titanium discs surfaces. Pseudopodia or apophysis of osteoblasts on TiO₂ blasted-etched titanium and Al₂O₃ blasted-etched titanium discs surfaces embedded into the first and second grade of pouches.4 The determination of proliferative activity of osteoblasts on three kinds of titanium surfacesComparisons of absorbance values of osteoblasts cultivated on three kinds of titanium surfaces for 3 days: absorbance values of osteoblasts on surfaces of TiO₂ blasted-etched titanium groups （0.380±0.018）, Al₂O₃ blasted-etched titanium groups （0.328±0.009） and smooth titanium groups （0.279±0.013） all had statistical differences （P< 0.05）.Conclusion:1 TiO₂ particle blasted-etched titanium surfaces, Al₂O₃ particle blasted-etched titanium surfaces and smooth titanium surfaces all had fine biological property and osteoblasts could well grow on three kinds of titanium discs surfaces;2 TiO₂ particle blasted-etched titanium surfaces and Al₂O₃ particle blasted-etched titanium surfaces were profit to the growth of osteoblasts and TiO₂ particle blasted-etched titanium surfaces were better to the growth of osteoblasts than Al₂O₃ particle blasted-etched titanium surfaces.

Related Dissertations

CLC: > Medicine, health > Oral Sciences > Oral orthotics > Dental Materials Science
© 2012 www.DissertationTopic.Net Mobile