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Effects of Tobacco of Human Osteoblasts Attachment and Proliferation on Titanium Implants

Author: ZhengYueHan
Tutor: LvChangSheng
School: Beijing Union Medical College
Course: Surgery
Keywords: Osteoblasts Tobacco infiltration of liquid Titanium
CLC: R783
Type: Master's thesis
Year: 2010
Downloads: 24
Quote: 0
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Abstract


Objective: Smoking on the various organs of the body will cause varying degrees of damage to the implant and the surrounding bone tissue formation , and thus hinder good osseointegration , to reduce implant treatment success rate by affecting bone metabolism . This paper tobacco infiltration with different concentrations of the liquid acting on the titanium plate attached osteoblasts , and observe its morphology , proliferative capacity , adhesion quantity, the change in the amount of alkaline phosphatase secreted at the cell level , understanding of harmful tobacco the direct impact of substances titanium plate - bone interface . Methods : tobacco with different concentrations of infiltration liquid ( smokingless tobacco extract ST ) role in the adhesion on the titanium plate osteoblast cell density and morphology changes observed under an inverted microscope , measured by MTT assay osteoblasts titanium plate adhesion number and proliferative capacity , and ALP (alkaline phosphatase, ALP) secretion was measured by the benzene disodium phosphate . Results: With the tobacco infiltration solution concentration increases , the cell range extending gradually narrowing shape gradually rendered as a long strip , even many cell rupture . Compared with the normal control group , the number of the adhesion of the cells on a titanium plate , proliferative capacity , and ALP secretion is proportional to the the infiltrating liquid concentration and its duration of action are subject to tobacco infiltration of liquid is suppressed, and the degree of suppression of tobacco . Conclusion: Smoking on the implant and the surrounding bone tissue formation have a negative impact on the good osseointegration , this effect is related to tobacco use time and concentration , is one of the important factors leading to implantation failure .

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