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The Effect of Ifenprodil on Focal Cerebral Ischemical Reperfusion Injury in Rats

Author: LiuXueJuan
Tutor: YangJinSheng
School: Lanzhou University
Course: Neurology
Keywords: Cerebral ischemia - reperfusion Survivin Ifenprodil Musashi1 Nerve regeneration
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 55
Quote: 0
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Abstract


The first part Ifenprodil on cerebral ischemia reperfusion survivin expression Objective: To observe the middle cerebral artery occlusion and reperfusion in rats ipsilateral hippocampus of Survivin Expression and ifenprodil on brain missing blood reperfusion time phases Survivin expression. Methods: 72 Wistar rats were randomly divided into sham operation group, model group, Ifenprodil group, the line tied embolization of focal cerebral ischemia model in rats, sham operation group in the postoperative, model group, Affin to Seoul in the intervention group, respectively, after cerebral ischemia and reperfusion 2h 3h, 24h, 72h, 7d were sacrificed, immunofluorescence measurements at different phases ischemic hippocampal neurons Survivin expression. Results: sham group showed basal rate Survivin expression in the model group, the intervention group Ifenprodil Survivin expression pattern consistent 3h reperfusion, expressed that there is increased to a peak 72h, 7d expression decreased, and false surgery group, P lt; 0.05; Ifenprodil fluorescence intensity intervention group was significantly higher than the corresponding model group (P lt; 0.05). Conclusions: 1. Middle cerebral artery occlusion and reperfusion increased expression of Survivin, 72h pm to peak; 2. Ifenprodil for middle cerebral artery ischemia-reperfusion injury in rats. The second part Ifenprodil on cerebral ischemia reperfusion rats Musashi1 Objective: To study Ifenprodil cerebral ischemia and reperfusion in rat brain tissue at different phases of the protective action of Musashil expression. Methods: 84 Wistar rats were randomly divided into three groups, sham operation group, cerebral ischemia-reperfusion model group, ifenprodil each group 28, with thread tied embolization middle cerebral artery occlusion model, respectively. reperfusion 2d, 3d, 7d, 14d rats were sacrificed 7, in which a nerve cell for TEM ultrastructure, 6 Nissl staining of histological changes, immunohistochemical studies Musashil expression pattern . Results: ① the sham operation group, model group has the following performance electron microscopy: Chromosome edge set, neuronal cell bodies and organelle swelling significantly, cytoplasmic density reduction; Nissl staining: the number of neurons decreased cell body swelling, Nissl body decreased or disappeared; immunohistochemistry: Musashil positive cells in reperfusion appears when a slight increase 2d, 3d, expressed significantly increased, 7d reached the peak, there is still a higher expression continued to 14d. ② Compared with the model group, the intervention group showed the following features, electron microscopy: organelle mild swelling; Nissl staining: Nissl bodies more; immunohistochemistry: Musashil positive cells compared to the same point in the model group was increased, the optical density values increased expression pattern with the model group. Conclusion: Ifenprodil cerebral ischemia and reperfusion can reduce cell damage caused, with the promotion of the expression of nerve regeneration factor Musashil role for improving cerebral ischemia-reperfusion injury has a positive effect.

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