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Objective Ulinastatin (ulinastatin, UTI) on severe acute pancreatitis (severe acute pancreatitis, SAP) rat brain capillary leakage effect and mechanism . Methods retrograde cholangiopancreatography injection of 5% sodium taurocholate SAP model , SAP molding femoral vein 5 minutes after injection Ulinastatin (10000IU/kg) as ulinastatin (UTI) intervention group , flip open the abdomen was closed immediately after the pancreas as the sham group (sham operation SO). 48 SD rats were randomly divided into the SO group, SAP group , UTI groups 6h and 24h is divided into two sub- groups, each sub-group 8 . Observed 6h, 24h mortality in each group after the serum TNF-α and pancreas, brain tissue pathology, wet and dry weight method detects brain water content , RT-PCR detection of occludin mRNA expression in brain tissue , brain tissue immunohistochemistry occludin expression . Results SO group, SAP group , UTI treatment group 6h serum TNF-α were (34.76 ± 4.44) pg / ml, (94.04 ± 4.53) pg / ml, (77.26 ± 4.32) pg / ml, 24h serum TNF-α were was (40.28 ± 2.16) pg / ml, (146.17 ± 4.89) pg / ml, (112.72 ± 4.77) pg / ml, differences between the groups were significantly (P lt; 0.05); 6h brain tissue water content were (71.11 ± 3.84)%, (79.99 ± 1.02)%, (78.02 ± 0.54)%, 24h brain tissue water content were (78.37 ± 0.50)%, (82.53 ± 0.90)%, (79.82 ± 0.71)%, differences between groups were significantly (P lt; 0.05); 6h occludin expression in brain tissue were 6.89 ± 0.34,3.95 ± 0.16,6.03 ± 0.24,24 h occludin expression in brain tissue were 7.88 ± 0.44,4.30 ± 0.54,6.34 ± 0.55, group differences were significant (P lt; 0.05); 6h brain occludinmRNA expression was 0.59 ± 0.07,0.35 ± 0.04,0.44 ± 0.04,24 h brain tissue occludinmRNA expression was 0.78 ± 0.06,0.60 ± 0.04,0.70 ± 0.03, group differences were significant (P lt; 0.05); SAP6h, 24h two subgroups of serum TNF-α, brain tissue water content compared with the SO group was significantly higher (P lt; 0.05), brain tissue occludinmRNA, brain tissue occludin protein expression compared with SO group was significantly decreased (P lt; 0.05); UTI treatment group 6h, 24h two subgroups of serum TNF-α, brain tissue water content, compared with SAP group was significantly decreased brain tissue occludinmRNA, brain tissue occludin protein expression compared with the SO group was significantly higher (P lt ; 0.05 ) ; Conclusion ulinastatin can reduce severe acute pancreatitis in rat brain capillary leak , possibly one of the mechanisms through reduced TNF-α, indirectly increase occludin expression .
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