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Objective: injection of hamster cheek pouch carcinoma model, the dynamic observation tumorigenesis process; explore the impact of carbon ion beam irradiation of the body trace elements, and apoptosis-related gene Survivin and Caspase-3 expression for heavy ion cancer therapy and space provide experimental evidence for radiation risk assessment. MATERIALS AND METHODS: This study selected 81 healthy golden hamsters were randomly divided into three groups, including blank control group (6), smear group (15), (60) (injection group was divided into A, B, , C, D, E5 groups). Using preset concentration (0.5%, 0.4%, 0.3%, 0.2%, 0.1%) DMBA acetone injection and smearing (0.5%) for the comparison of hamster cheek pouch carcinoma model, histological morphological analysis, ways to different pathological stage in the carcinogenesis process. Gross observation, monitoring weight and calculate the similarities and differences between the tumor growth rate, screening the appropriate concentration of the injection to establish an animal model of oral squamous cell carcinoma. Carbon ion beam irradiation Dutch cheek pouch carcinoma of hamster, observing of 0,4,6,8,12 Gy dose of heavy ion beam on the content of trace elements in the bearing cheek pouch carcinoma of hamster blood, the application of atomic absorption The Spectrometer flame Determination serum iron (Fe), copper (Cu), zinc (Zn), magnesium (Mg), calcium (Ca) content of microelements. Using immunohistochemical SP method detected 40 cases of the Dutch golden hamster cheek pouch carcinoma tissue Survivin and Caspase-3 protein expression, and to explore the impact of the heavy ion beam irradiation of the tumor tissue. Results: 1. Injection concentration group was 0.4%, 0.3%, 0.2% and 0.1%, respectively in 13W, 9W, 12W, 16W, the size of the tumor is about 0.8 × 0.6cm × 0.5cm; 12W into cancer rates were 25 %, 87%, 42%, 58%; 0.5% DMBA acetone smear group, 12W into cancer rate was 48%, the mortality rate was 37%, buccal 18W when well-differentiated squamous cell carcinoma. Golden hamster tumor Trace Fe, Zn, Cu, Mg element content in serum was significantly lower than the normal group (P lt; 0.05); then through the heavy ion beam irradiation of different doses of January, serum five trace elements in the low dose showed a downward trend, and appears elevated 8Gy was decreased, there is a certain dose - response relationship. 3. Survivin expression in the golden hamster buccal squamous cell carcinoma (OSCC) positive in 28 cases (28/40) to zero (0/8) expression in normal oral mucosa (NOM); of Caspase-3 in the golden hamster buccal squamous cell carcinoma (OSCC) positive expression in 30 cases (30/40) in normal oral mucosa (NOM), 100% (8/8); After statistical analysis, Survivin and Caspase-3 in OSCC expression was statistically significant (P <0.05), (12) C 6 ion irradiation the OSCC in Survivin expression was statistically significant (P <0.05), Caspase -3 expression was not statistically significant (P gt; 0.05), during the irradiation and tumor clinical classification was not statistically significant (P <0.05). Conclusion: 1. Were screened with 0.3% DMBA acetone liquid injection method can be induced to form an animal model of oral squamous cell carcinoma, and shorten the experimental period, reduce the cost of modeling. Different doses of heavy ion beam local irradiation of tumor-bearing golden hamster will affect the content of trace elements in the serum of some clinical significance. 3. (12) C 6 ion irradiation in oral squamous cell carcinoma Survivin and Caspase-3 protein expression have an impact down the expression of Survivin indirect regulation of Caspase-3 activity involved in the process of apoptosis regulation.
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